Active Recombinant Human IFNAR1 and IFNAR2 Protein, His-tagged

• Cat.No.: IFNAR1-70H
• Product Overview: IFNAR (IFNAR1/R2) heterodimer protein contains IFNAR1 and IFNAR2 extracellular domains (UniProt# P17181 and UniProt# P48551) fused with a proprietary dimer motif followed by a His tag at each C-termini.

Specification

• Species: Human
• Source: HEK293T
• Tag: His
• Protein Length: IFNAR1: 27-243 aa IFNAR2: 28-436 aa
• Description: The interferon-alpha/beta receptor (interferon-α/β receptor, IFNAR, IFNaR, IFNαR), also known as Type 1 Interferon Receptor, is primarily located on the cell plasma membrane. IFNAR is a heterodimeric cell surface receptor complex composed of two subunits, IFNAR1 and IFNR2. Each subunit of IFNAR contains an N-terminal ligand binding domain containing two (IFNAR2) or four (IFNAR1) fibronectin type II-like subdomains, a transmembrane domain, and a cytoplasmic domain. IFNAR1 and IFNAR2 association is essential for recognizing type I interferon (IFN) cytokines such as IFN-α, IFN-β and others. The extracellular domains (ECDs) of IFNAR1 and IFNAR2 are specifically responsible for binding Type 1 interferons. Type 1 interferon interaction with both IFNAR1 and IFNAR2 is essential for stabilizing the receptor-ligand binding and facilitating downstream signaling. Upon binding to type I IFNs, IFNAR activates the JAK-STAT, MAPK, PI3K, and Akt signaling pathways. As Type 1 interferon signaling plays important roles in immune responses, dysregulation of IFNAR activated pathways has been associated with various diseases, such as neurodegenerative diseases, autoimmune disorders and cancer. IFNAR is an emerging therapeutic target for various types of diseases involving modulation of Type I interferon signaling. Our recombinant IFNAR1/R2 protein features the IFNAR heterodimer of IFNAR1 and IFNAR2.
• Molecular Mass: 87 kDa
• Homodimer/Heterodimer: Heterodimer
• Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
• Application: Verified Applications: ELISA, SPR, and BLI for IFNAR-specific antibody assays; ELISA, SPR, and BLI for type I interferon (IFN) cytokine ligand/receptor protein binding assays. Suggested Applications: ELISA for IFNAR-specific antibody binding assays; ELISA for type I interferon (IFN) cytokine ligand/receptor protein binding assays. SPR & BLI for IFNAR-specific antibody binding assays; SPR & BLI for type I interferon (IFN) cytokine ligand/receptor protein binding assays. Animal immunization, RUO.
• Storage: At -80 centigrade
• Storage Buffer: 0.22μm filtered PBS, pH 7.4
• Shipping: Frozen Dry Ice

Gene Information

• Gene Name 2: IFNAR1 interferon (alpha, beta and omega) receptor 1 [Homo sapiens (human)]
• Official Symbol 2: IFNAR1
• Synonyms 2: IFNAR1; interferon (alpha, beta and omega) receptor 1; IFNAR; interferon alpha/beta receptor 1; IFRC; CRF2-1; IFN-R-1; IFN-alpha/beta receptor 1; interferon-beta receptor 1; beta-type antiviral protein; alpha-type antiviral protein; type I interferon receptor 1; cytokine receptor class-II member 1; cytokine receptor family 2 member 1; interferon-alpha/beta receptor alpha chain; AVP; IFNBR; IFN-alpha-REC
• Gene ID 2: 3454
• mRNA Refseq 2: NM_000629
• Protein Refseq 2: NP_000620
• MIM 2: 107450
• UniProt ID 2: P17181
• Gene Name: IFNAR2 interferon (alpha, beta and omega) receptor 2 [Homo sapiens (human)]
• Official Symbol: IFNAR2
• Synonyms: IFNAR2; interferon (alpha, beta and omega) receptor 2; IFNABR; interferon alpha/beta receptor 2; IFN-R-2; IFN-alpha binding protein; IFN-alpha/beta receptor 2; type I interferon receptor 2; interferon alpha binding protein; human interferon alpha/beta receptor; interferon-alpha/beta receptor beta chain; IFN-R; IFNARB; IFN-alpha-REC
• Gene ID: 3455
• mRNA Refseq: NM_000874
• Protein Refseq: NP_000865
• MIM: 602376
• UniProt ID: P48551