Active Recombinant Human IL6 Protein (185 aa)

• Cat.No.: IL6-351I
• Product Overview: Recombinant human Interleukin-6 (rhIL-6) produced in E. coli is a single non-glycosylated polypeptide chain containing 185 amino acids. A fully biologically active molecule, rhIL-6 has a molecular mass of 20.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.

Specification

• Description: Interleukin-6 (IL-6, also known as IFN-β2) is a pleiotropic cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 is secreted by T cells and macrophages to stimulate immune response. IL-6 is responsible for stimulating acute phase protein synthesis, as well as the production of neutrophils in the bone marrow. It supports the growth of B cells and is antagonistic to regulatory T cells.
• Source: E. coli
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 0.1 ng/mL, measured by the dose-dependent stimulation of the proliferation of IL-6 dependent murine 7TD1 cells, corresponding to a specific activity of > 1 × 10^7 units/mg.
• Molecular Mass: 20.9 kDa, observed by reducing SDS-PAGE.
• Protein length: 185
• AA Sequence: MPVPPGEDSKDVAAPHRQPLTSSER IDKQIRYILDGISALRKETCNKSNM CESSKEALAENNLNLPKMAEKDGCF QSGFNEETCLVKIITGLLEFEVYLE YLQNRFESSEEQARAVQMSTKVLIQ FLQKKAKNLDAITTPDPTTNASLLT KLQAQNQWLQDMTTHLILRSFKEFL QSSLRALRQM
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% by SDS-PAGE analysis.
• Storage: Lyophilized recombinant human Interleukin-6 (rhIL-6) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhIL-6 should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against 1xPBS.
• Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Gene Information

• Gene Name: IL6 interleukin 6 (interferon, beta 2) [ Homo sapiens ]
• Official Symbol: IL6
• Synonyms: IL6; interleukin 6 (interferon, beta 2); IFNB2; interleukin-6; BSF2; HGF; HSF; IL 6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor; IL-6
• Gene ID: 3569
• mRNA Refseq: NM_000600
• Protein Refseq: NP_000591
• MIM: 147620
• UniProt ID: P05231

Reviews

03/05/2022

Accurate localization and function within the cell.

01/31/2021

The binding affinity and affinity constant with the target are high.

03/12/2020

Can form a stable micellar structure.

Q&As

What is the dosage and usage of Il6?

The dosage and usage of Il6 will vary according to the disease, and generally require individualized regulation by the physician according to the disease situation.

What are the pharmacokinetic properties of Il6?

The pharmacokinetic characteristics of Il6 mainly include drug absorption, distribution, metabolism, excretion and other information.

Does Il6 have advantages in the treatment of multiple myeloma?

Recombinant Il6 protein has a unique therapeutic advantage in the treatment of multiple myeloma. It can promote the proliferation and differentiation of hematopoietic stem cells and inhibit the growth of tumor cells.

Does Il6 have specific therapeutic effects for certain diseases?

Il6 has specific therapeutic effects for certain diseases, such as rheumatoid arthritis, multiple myeloma and so on.

In which diseases is Il6 widely used?

This protein is widely used in the treatment of rheumatoid arthritis, pneumonia, multiple myeloma, lymphoma, malignant tumors and other diseases.

What is the effect of Il6 on immune cells?

The effects of Il6 on immune cells are mainly manifested as promoting cell proliferation and differentiation, regulating immune response, etc.