Active Recombinant Human Killer Cell Immunoglobulin-Like Receptor, Three Domains, Short Cytoplasmic Tail, 1, Fc-tagged

• Cat.No.: KIR3DS1-5670H
• Product Overview: RecombinantHuman KIR3DS1protein was expressed in Chinese HamsterOvary cell line with the Fc part of Human IgG1 Pro100-Lys330(C-terminus) and Human KIR3DS1 His22-His340 (N-terminus).

Specification

• Species: Human
• Source: CHO
• Tag: Fc
• Protein Length: 22-340 a.a.
• Description: KIR3DS1(3DS1, CD158e2) is a type I transmembrane protein that belongs to the killercell Ig-like receptor (KIR) family. KIRs are expressed on CD56 dim NK cellsand T cell subsets where they differentiate normal from abnormal cells, and regulateeffector functions in the innate immune system. KIRs are named for the numberof Ig-like domains (2D or 3D) in the extracellular domain(ECD), and whether theyhave long or short (L, S) cytoplasmic tails. Like other activating KIRs, KIR3DS1has a short cytoplasmic tail and a positively charged amino acid (aa) withinthe transmembrane domain that interacts with the ITAM-bearing signalingadaptor, DAP12.
• Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS.
• N-terminalSequence: His22
• MolecularWeight: 61.9 kDa (monomer)
• Activity: Measuredby its ability to bind MDA-MB-231 human breast adenocarcinoma cells in a flowcytometry assay. A 200-500 fold increase in fluorescence intensity can be observedwhen MDA-MB-231 cells are stained with 3 μg/mL rhKIR3DS1/Fc Chimera and goatanti-human Fc-PE as compared to cells stained with goat anti-human Fc-PE.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 75-90kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: KIR3DS1 killer cellimmunoglobulin-like receptor, three domains, short cytoplasmic tail, 1 [ Homosapiens ]
• Official Symbol: KIR3DS1
• Synonyms: KIR3DS1; killer cellimmunoglobulin-like receptor, three domains, short cytoplasmic tail, 1; KIR-G1; NKAT10; CD158E2; KIR-123FM; killercell immunoglobulin-like receptor 3DS1; NKAT-10; killer Ig receptor; MHCclass I NK cell receptor; natural killer-associated transcript 10; killercell immunoglobulin-like receptor KIR3DS1
• Gene ID: 3813
• mRNA Refseq: NM_001083539
• Protein Refseq: NP_001077008
• UniProt ID: Q14943
• Chromosome Location: 19q13.4
• Function: MHC class I receptor activity ; receptoractivity