Active Recombinant Human LGMN, His-tagged
| Cat.No. : | LGMN-7980H |
| Product Overview : | Recombinant Human LGMN (Ile18-Tyr433) fused with an N-terminal 7-His tag, was expressed in Mouse NSO Cells. |
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| Species : | Human |
| Source : | Mammalian Cells |
| Tag : | His |
| Protein Length : | 18-433 a.a. |
| Description : | This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. |
| Predicted N Terminal : | His |
| Form : | Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol. |
| Bio-activity : | Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >250 pmol/min/µg. |
| Molecular Mass : | Predicted Molecular Mass:49 kDa SDS-PAGE:60 kDa, reducing conditions |
| Endotoxin : | < 1.0 EU per 1 μg of the protein by the LAL method. |
| Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Storage : | Avoid repeated freeze-thaw cycles. 3 months from date of receipt, -70 °C as supplied. 1 month, -70 °C under sterile conditions after opening. |
| Gene Name | LGMN legumain [ Homo sapiens (human) ] |
| Official Symbol | LGMN |
| Synonyms | LGMN; AEP; LGMN1; PRSC1; legumain; cysteine protease 1; protease, cysteine 1; asparaginyl endopeptidase; protease, cysteine, 1 (legumain); EC 3.4.22.34 |
| Gene ID | 5641 |
| mRNA Refseq | NM_005606 |
| Protein Refseq | NP_005597 |
| MIM | 602620 |
| UniProt ID | Q99538 |
| Chromosome Location | 14q32.1 |
| Pathway | Adaptive Immune System; Antigen processing and presentation; Immune System |
| Function | cysteine-type endopeptidase activity; peptidase activity |
Not For Human Consumption!
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