Active Recombinant Human MAP3K10(1-449) Protein, N-His-tagged

Cat.No. : MAP3K10-153H
Product Overview : N-terminal 6Histagged, recombinant, human MLK2, amino acids 1-449 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
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Description : MLK2 is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phosphorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis.
Source : Sf21
Species : Human
Tag : His
Protein length : 1-449 aa
Form : 0.65mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Bio-activity : 532U/mg, where one unit of MLK2 active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
Molecular Mass : 54 kDa
AA Sequence : 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MEEEEGAVAK EWGTTPAGPV WTAVFDYEAA 61 GDEELTLRRG DRVQVLSQDC AVSGDEGWWT GQLPSGRVGV FPSNYVAPGA PAAPAGLQLP 121 QEIPFHELQL EEIIGVGGFG KVYRALWRGE EVAVKAARLD PEKDPAVTAE QVCQEARLFG 181 ALQHPNIIAL RGACLNPPHL CLVMEYARGG ALSRVLAGRR VPPHVLVNWA VQVARGMNYL 241 HNDAPVPIIH RDLKSINILI LEAIENHNLA DTVLKITDFG LAREWHKTTK MSAAGTYAWM 301 APEVIRLSLF SKSSDVWSFG VLLWELLTGE VPYREIDALA VAYGVAMNKL TLPIPSTCPE 361 PFARLLEECW DPDPHGRPDF GSILKRLEVI EQSALFQMPL ESFHSLQEDW KLEIQHMFDD 421 LRTKEKELRS REEELLRAAQ EQRFQEEQLR RREQELAERE MDIVERELHL LMCQLSQEK
Purity : Purity 82% by SDS-PAGE and Coomassie blue staining.
Applications : Drug Discovery & Development
Storage : 1 year at -70 centigrade.
Handling Advice : Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
Use/Stability : On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.
SDS-PAGE: 1.2–25ng of this lot of enzyme phosphorylated 0.33mg/ml myelin basic protein in the assay.
Gene Name : MAP3K10 mitogen-activated protein kinase kinase kinase 10 [ Homo sapiens (human) ]
Official Symbol : MAP3K10
Synonyms : MAP3K10; mitogen-activated protein kinase kinase kinase 10; MST; MLK2; MEKK10
Gene ID : 4294
mRNA Refseq : NM_002446.4
Protein Refseq : NP_002437.2 
MIM : 600137
UniProt ID : Q02779

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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