Recombinant Human Mitogen-Activated Protein Kinase Kinase Kinase 10, GST-Tagged

• Cat.No.: MAP3K10-1038H
• Product Overview: Recombinant human MAP3K10 (1-446) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: MAP3K10-1038H
• Description: MAP3K10 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8 JNK and MKK4/SEK1. MAP3K10 itself can be phoshorylated, and activated by JNK kinases. MAP3K10 is also known as mitogen-activated protein kinase kinase kinase 10 (MAP3K10). MAP3K10 functions preferentially on the JNK signaling pathway which is involved in nerve growth factor (NGF) induced neuronal apoptosis. MAP3K10 was isolated as a cDNA fragment from MKN28 gastric cancer cell library using degenerate PCR. MAP3K10 is highly expressed in brain, skeletal muscle, and testis.
• Applications: Kinase Assay, Western Blot
• Molecular Weight: 76 kDa
• Expression System: Sf9 insect cells using baculovirus.
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
• Specific Activity: 948 nmol/min/mg
• Purity: > 85 %
• Concentration: 0.1 ug/ul
• Sequences: 1-446
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Insulin Signaling; p38 MAPK signaling pathwa

Gene Information

• Gene Name: MAP3K10 mitogen-activated protein kinase kinase kinase 10 [ Homo sapiens ]
• Official Symbol: MAP3K10
• Synonyms: MAP3K10; MST; MLK2; MEKK10; mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; Protein kinase MST; EC 2.7.11.25; MKN28 derived nonreceptor_type serine/threonine kinase; MKN28 kinase; EC 2.7.11
• Gene ID: 4294
• mRNA Refseq: NM_002446
• Protein Refseq: NP_002437
• MIM: 600137
• UniProt ID: Q02779
• Chromosome Location: 19q13.2
• Function: ATP binding; JUN kinase kinase kinase activity; MAP kinase kinase activity; nucleotide binding; protein homo- dimerization activity; protein serine/threonine kinase activity; protein tyrosine kinase activity