Active Recombinant Human NRAS Protein, His-tagged

Cat.No. : NRAS-01H
Product Overview : The wild-type human N-Ras protein has been produced in a bacterial expression system. The recombinant protein contains six histidine residues at its amino terminus (His-tag).
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Description : This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
Source : Bacterial expression system
Species : Human
Tag : His
Form : White lyophilized powder
Bio-activity : The biological activity of N-Ras can be determined from the ability of the RasGRF1 exchange domain (Ras-GRF1-ExD) to catalyze the exchange of GDP for GTP on N-Ras. A standard biological assay for monitoring the biological activity of N-Ras is an exchange assay utilizing the 2× Exchange Buffer from the RhoGEF exchange assay kit and the human RasGRF GEF domain.
Molecular Mass : 25 kDa
Purity : Protein purity is determined by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gradient gel. His tagged N-Ras protein was determined to be > 90% pure.
Applications : Study of N-Ras exchange activity with different GEFs, Identification of N-Ras exchange factors (GEFs), Positive control for GEF studies, Biochemical characterization of N-Ras protein interactions, Western blot standard
Stability : The protein is stable for six months if stored at -70 centigrade. The protein should not be exposed to repeated freezethaw cycles. The lyophilized protein is stable at 4 centigrade desiccated (<10% humidity) for one year.
Storage : In order to maintain high biological activity of the protein, it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration, aliquoted into "experiment-sized" amounts, snap frozen in liquid nitrogen, and stored at -70 centigrade.
Reconstitution : Before reconstitution, briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 5 mg/mL with the addition of 20 μL of Milli-Q water (100 μg size). When reconstituted, the protein will be in the following buffer: 50 mM Tris pH 7.5, 50 mM NaCl, 0.5 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran.
SDS-PAGE: A 20 μg sample of recombinant N-Ras protein (molecular weight approx. 25 kDa) was separated by electrophoresis in a 4-20% SDS-PAGE system and stained with Coomassie Blue. Protein quantitation was determined using the Precision Red Protein Assay Reagent. Mark12 molecular weight markers are from Life Technologies Inc.
Gene Name : NRAS NRAS proto-oncogene, GTPase [ Homo sapiens (human) ]
Official Symbol : NRAS
Synonyms : NRAS; NRAS proto-oncogene, GTPase; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1; GTPase NRas; N-ras protein part 4; neuroblastoma RAS viral (v-ras) oncogene homolog; neuroblastoma RAS viral oncogene homolog; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog; EC 3.6.5.2
Gene ID : 4893
mRNA Refseq : NM_002524
Protein Refseq : NP_002515
MIM : 164790
UniProt ID : P01111

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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