Active Recombinant Human PHGDH Protein, His-tagged

• Cat.No.: PHGDH-01H
• Product Overview: Recombinant Human PHGDH (phosphoglycerate dehydrogenase), full length, encompassing amino acids 1-533(end). This construct contains a C-terminal His-tag (6xHis). The recombinant protein was affinity purified.

Specification

• Description: This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Aqueous buffer solution
• Bio-activity: ≥80 pmol/min/μg
• Molecular Mass: 57.5 kDa
• Protein length: 1-533
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Usage: The reaction mixture contained 100 mM Tris-HCl, pH 8.8, 400 mM NaCl, 0.2 mM DTT, 2.5 mM 3-phospho-D-glyceric acid (Sigma P8877), 1 mM NAD, and various amount of PHGDH in a final volume of 50 μL. Reactions were initiated with the addition of PHGDH, and the increase in NADH fluorescence (Ex340/Em460 nm) was continuously monitored for 10 min at room temperature.
• Storage: >6 months at –80 centigrade
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, variable imidazole, 0.04% Tween-20, and 20% glycerol.
• Shipping: At -80 centigrade
• Warning: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: PHGDH phosphoglycerate dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: PHGDH
• Synonyms: PHGDH; phosphoglycerate dehydrogenase; NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113; D-3-phosphoglycerate dehydrogenase; 2-oxoglutarate reductase; 3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113; malate dehydrogenase; EC 1.1.1.37; EC 1.1.1.399; EC 1.1.1.95
• Gene ID: 26227
• mRNA Refseq: NM_006623
• Protein Refseq: NP_006614
• MIM: 606879
• UniProt ID: O43175