Recombinant Human PHGDH protein, His-tagged

• Cat.No.: PHGDH-3530H
• Product Overview: Recombinant Human PHGDH protein(185-533 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 185-533 aa
• AA Sequence: SFGVQQLPLEEIWPLCDFITVHTPL LPSTTGLLNDNTFAQCKKGVRVVNC ARGGIVDEGALLRALQSGQCAGAAL DVFTEEPPRDRALVDHENVISCPHL GASTKEAQSRCGEEIAVQFVDMVKG KSLTGVVNAQALTSAFSPHTKPWIG LAEALGTLMRAWAGSPKGTIQVITQ GTSLKNAGNCLSPAVIVGLLKEASK QADVNLVNAKLLVKEAGLNVTTSHS PAAPGEQGFGECLLAVALAGAPYQA VGLVQGTTPVLQGLNGAVFRPEVPL RRDLPLLLFRTQTSDPAMLPTMIGL LAEAGVRLLSYQTSLVSDGETWHVM GISSLLPSLEAWKQHVTEAFQFHF
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: PHGDH phosphoglycerate dehydrogenase [ Homo sapiens ]
• Official Symbol: PHGDH
• Synonyms: PHGDH; phosphoglycerate dehydrogenase; D-3-phosphoglycerate dehydrogenase; PDG; PGDH; SERA; 3-phosphoglycerate dehydrogenase; PGD; PGAD; 3PGDH; 3-PGDH; MGC3017
• Gene ID: 26227
• mRNA Refseq: NM_006623
• Protein Refseq: NP_006614
• MIM: 606879
• UniProt ID: O43175