Species : |
Human |
Source : |
E.coli |
Tag : |
His |
Description : |
Ran (TC4), a small GTP binding protein, is the heart of the nuclear transport process. It is involved in the directionality of the process that is regulated by GTP hydrolysis. Ran shuttles between the nucleus and the cytoplasm. In the nucleus, it exists in Ran-GTP form, which is maintained by the Ran-Guanine nucleotide exchange protein, RCC1, a nucleus specific, chromatin bound protein. Ran-GTP forms complexes with transport proteins that shuttle from the nucleus to the cytoplasm i.e. improtin β, CRM1/exportin1. In the cytoplasm, Ran intrinsic GTPase activity is stimulated by Ran-GAP causing the hydrolysis of GTP to GDP and disassembly of the resulted Ran-GDP from the transport protein. Ran-GDP reenters the nucleus by active transport as NTF2/Ran-GDP complex. |
Form : |
Solution in 20 mM HEPES-KOH, 110 mM potassium acetate, 2 mM magnesium acetate, 0.5 mM EGTA, 2 mM DTT, and 5% glycerol, pH 7.3. |
Bio-activity : |
Binds GDP at more than 0.1 molar ratio. |
Purity : |
>90% (SDS-PAGE) |
Applications : |
Ran, N-terminal histidine tagged, is a 26.6 kDa protein. It appears on SDS-PAGE as a 31 kDa protein in low ionic strength buffer. In high ionic strength buffers, it appears as a ladder of bands probably representing oligomerization. Ran was purified in the presence of GDP. |
Storage : |
Store product at -70 centigrade. |