Active Recombinant Human SRC(1-530) Protein, N-His-tagged

• Cat.No.: SRC-231H
• Product Overview: N-terminal 6His-tagged, recombinant, human Src, amino acids 1–530, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose. The mutant form of Src (Src, 1–530) has been identified in cases of advanced colon cancer. This truncating mutation generates an open active form of Src that has been found to be transforming, tumorigenic and metastasis promoting.

Specification

• Description: Src family tyrosine kinases are non-receptor tyrosine kinases that are functionally linked to some receptor-type tyrosine kinases, G-protein-coupled receptors, cytokine receptors, and cell adhesion molecules/receptors. Src family tyrosine kinases are membrane-associated proteins having Src homology 2 (SH2) and SH3 domains, which are important for protein-protein interaction. The activity of Src family tyrosine kinases is regulated through intramolecular interaction between the SH2 domain and the phosphorylated tyrosine at the C-terminal regulatory site (Tyr-527).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-530 aa
• Form: 2.265mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1956U/mg, where one unit of Src (1–530) activity is defined as 1nmol phosphate incorporated into 500μM (GGEEEEYFELVKKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 61.1 kDa
• AA Sequence: 1 MSYYHHHHHH DEFMGSNKSK PKDASQRRRS LEPAENVHGA GGGAFPASQT PSKPASADGH 61 RGPSAAFAPA AAEPKLFGGF NSSDTVTSPQ RAGPLAGGVT TFVALYDYES RTETDLSFKK 121 GERLQIVNNT EGDWWLAHSL STGQTGYIPS NYVAPSDSIQ AEEWYFGKIT RRESERLLLN 181 AENPRGTFLV RESETTKGAY CLSVSDFDNA KGLNVKHYKI RKLDSGGFYI TSRTQFNSLQ 241 QLVAYYSKHA DGLCHRLTTV CPTSKPQTQG LAKDAWEIPR ESLRLEVKLG QGCFGEVWMG 301 TWNGTTRVAI KTLKPGTMSP EAFLQEAQVM KKLRHEKLVQ LYAVVSEEPI YIVTEYMSKG 361 SLLDFLKGET GKYLRLPQLV DMAAQIASGM AYVERMNYVH RDLRAANILV GENLVCKVAD 421 FGLARLIEDN EYTARQGAKF PIKWTAPEAA LYGRFTIKSD VWSFGILLTE LTTKGRVPYP 481 GMVNREVLDQ VERGYRMPCP PECPESLHDL MCQCWRKEPE ERPTFEYLQA FLEDYFTSTE 541 PQY
• Purity: Purity 100% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: SRC
• Synonyms: SRC; SRC proto-oncogene, non-receptor tyrosine kinase; ASV; SRC1; THC6; c-SRC; p60-Src
• Gene ID: 6714
• mRNA Refseq: NM_005417.5
• Protein Refseq: NP_005408.1
• MIM: 190090
• UniProt ID: P12931

Reviews

01/01/2022

Top-notch protein purification service, essential for our research.

08/29/2019

Reliable mass spectrometry analysis, pivotal for our proteomics work.

03/02/2019

Swift and reliable protein sequencing, indispensable for our research success.

Q&As

How do alterations in SRC expression or activity affect the development of drug resistance in tumors?

Changes in SRC expression or kinase activity can lead to drug resistance in cancer treatment.

How does SRC (proto-oncogene tyrosine-protein kinase Src) regulate cell proliferation and survival?

SRC regulates cell proliferation and survival through its kinase activity, influencing various signaling pathways.

How does SRC contribute to the regulation of bone remodeling and osteoclast activity?

SRC is involved in bone remodeling, regulating osteoclast activity and bone resorption.

What role does SRC play in cancer development and progression, particularly in metastasis?

SRC plays a crucial role in cancer progression, particularly in enhancing metastatic potential of tumor cells.

How does SRC influence cellular signaling pathways, such as the Ras/MAPK and PI3K/Akt pathways?

SRC influences key cellular signaling pathways like Ras/MAPK and PI3K/Akt, affecting cell growth and survival.

What are the mechanisms by which SRC modulates cellular adhesion and migration?

SRC modulates cellular adhesion and migration, playing a role in tissue repair and cancer metastasis.

What is the impact of SRC inhibition on targeted cancer therapies?

SRC inhibition is a target in cancer therapy, potentially reducing tumor growth and metastasis.