Active Recombinant Human SRC Protein (Full Length), N-FLAG

• Cat.No.: SRC-10H
• Product Overview: Recombinant human SRC protein was expressed in a baculovirus expression system as the full length protein (accession number NP_005408.1) with an N-terminal FLAG tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag
• Protein Length: Full Length
• Description: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
• Bio-activity: 1 µM TK substrate was incubated with different concentrations of SRC protein in a reaction system containing 1× Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 μM ATP for 1 hour. The 10 μL detection reagents containing TK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer were added and incubated with the reactions for 1 hour. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.
• Molecular Mass: 61.1 kDa
• Purity: > 95%
• Applications: This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
• Notes: This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.25 μg/μL
• Storage Buffer: 25 mM HEPES-NaOH pH7.5,300 mM NaCl,10% glycerol,0.04% Triton X-100 and 0.5 mM TCEP.

Gene Information

• Gene Name: SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: SRC
• Synonyms: SRC; SRC proto-oncogene, non-receptor tyrosine kinase; ASV; SRC1; THC6; c-SRC; p60-Src; proto-oncogene tyrosine-protein kinase Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; EC 2.7.10.2
• Gene ID: 6714
• mRNA Refseq: NM_005417
• Protein Refseq: NP_005408
• MIM: 190090
• UniProt ID: P12931