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Active Recombinant Human SRC Protein (Full Length), N-FLAG

Cat.No. : SRC-10H
Product Overview : Recombinant human SRC protein was expressed in a baculovirus expression system as the full length protein (accession number NP_005408.1) with an N-terminal FLAG tag.
  • Specification
  • Gene Information
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Description : This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Source : Baculovirus
Species : Human
Tag : N-FLAG
Bio-activity : 1 µM TK substrate was incubated with different concentrations of SRC protein in a reaction system containing 1× Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 μM ATP for 1 hour. The 10 μL detection reagents containing TK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer were added and incubated with the reactions for 1 hour. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.
Molecular Mass : 61.1 kDa
Protein Length : Full Length
Purity : > 95%
Applications : This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Notes : This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Storage : Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Concentration : 0.25 μg/μL
Storage Buffer : 25 mM HEPES-NaOH pH7.5,300 mM NaCl,10% glycerol,0.04% Triton X-100 and 0.5 mM TCEP.
Gene Name : SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens (human) ]
Official Symbol : SRC
Synonyms : SRC; SRC proto-oncogene, non-receptor tyrosine kinase; ASV; SRC1; THC6; c-SRC; p60-Src; proto-oncogene tyrosine-protein kinase Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; EC 2.7.10.2
Gene ID : 6714
mRNA Refseq : NM_005417
Protein Refseq : NP_005408
MIM : 190090
UniProt ID : P12931

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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