Species : |
Human |
Source : |
Insect Cells |
Tag : |
Flag |
Protein Length : |
Full Length |
Description : |
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. |
Bio-activity : |
1 µM TK substrate was incubated with different concentrations of SRC protein in a reaction system containing 1× Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 μM ATP for 1 hour. The 10 μL detection reagents containing TK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer were added and incubated with the reactions for 1 hour. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity. |
Molecular Mass : |
61.1 kDa |
Purity : |
> 95% |
Applications : |
This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
Notes : |
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival. |
Storage : |
Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
Concentration : |
0.25 μg/μL |
Storage Buffer : |
25 mM HEPES-NaOH pH7.5,300 mM NaCl,10% glycerol,0.04% Triton X-100 and 0.5 mM TCEP. |