Active Recombinant Human SRC Protein (Full Length), N-FLAG
Cat.No. : | SRC-10H |
Product Overview : | Recombinant human SRC protein was expressed in a baculovirus expression system as the full length protein (accession number NP_005408.1) with an N-terminal FLAG tag. |
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Description : | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. |
Source : | Baculovirus |
Species : | Human |
Tag : | Flag |
Bio-activity : | 1 µM TK substrate was incubated with different concentrations of SRC protein in a reaction system containing 1× Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 μM ATP for 1 hour. The 10 μL detection reagents containing TK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer were added and incubated with the reactions for 1 hour. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity. |
Molecular Mass : | 61.1 kDa |
Protein Length : | Full Length |
Purity : | > 95% |
Applications : | This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
Notes : | This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival. |
Storage : | Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
Concentration : | 0.25 μg/μL |
Storage Buffer : | 25 mM HEPES-NaOH pH7.5,300 mM NaCl,10% glycerol,0.04% Triton X-100 and 0.5 mM TCEP. |
Gene Name : | SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens (human) ] |
Official Symbol : | SRC |
Synonyms : | SRC; SRC proto-oncogene, non-receptor tyrosine kinase; ASV; SRC1; THC6; c-SRC; p60-Src; proto-oncogene tyrosine-protein kinase Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; EC 2.7.10.2 |
Gene ID : | 6714 |
mRNA Refseq : | NM_005417 |
Protein Refseq : | NP_005408 |
MIM : | 190090 |
UniProt ID : | P12931 |
Products Types
◆ Recombinant Protein | ||
SRC-4276R | Recombinant Rhesus Macaque SRC Protein, His (Fc)-Avi-tagged | +Inquiry |
Src-1368M | Recombinant Mus musculus Src Protein (V85-L535) | +Inquiry |
SRC-0403H | Recombinant Human SRC Protein (G2-L536), His/Flag tagged | +Inquiry |
Src-907M | Active Recombinant Mouse Src protein(Met1-Leu535), His&GST-tagged | +Inquiry |
SRC-0402H | Recombinant Human SRC Protein (G2-L536), Tag Free | +Inquiry |
◆ Native Protein | ||
SRC-29697TH | Native Human SRC | +Inquiry |
◆ Lysates | ||
SRC-585MCL | Recombinant Mouse SRC cell lysate | +Inquiry |
SRC-487HCL | Recombinant Human SRC cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionChanges in SRC expression or kinase activity can lead to drug resistance in cancer treatment.
SRC regulates cell proliferation and survival through its kinase activity, influencing various signaling pathways.
SRC is involved in bone remodeling, regulating osteoclast activity and bone resorption.
SRC plays a crucial role in cancer progression, particularly in enhancing metastatic potential of tumor cells.
SRC influences key cellular signaling pathways like Ras/MAPK and PI3K/Akt, affecting cell growth and survival.
SRC modulates cellular adhesion and migration, playing a role in tissue repair and cancer metastasis.
SRC inhibition is a target in cancer therapy, potentially reducing tumor growth and metastasis.
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