Active Recombinant Human TNFRSF17 protein(Met1-Ala54), rFc-tagged

• Cat.No.: TNFRSF17-237H
• Product Overview: Recombinant Human TNFRSF17 (NP_001183.2) (Met1-Ala54) was expressed in HEK293 with the Fc region of Rabbit IgG at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: rFc
• Protein Length: Met1-Ala54
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized TNFRSF17 at 10 μg/mL (100 μL/well) can bind Fc-BAFF/Biotin, the EC50 of Fc-BAFF/Biotin is 1.0-10.0 ng/mL.
• Molecular Mass: The recombinant human TNFRSF17 consists 303 amino acids and predicts a molecular mass of 33.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human TNFRSF17 is approximately 33-44 kDa due to the glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF17 tumor necrosis factor receptor superfamily, member 17 [ Homo sapiens ]
• Official Symbol: TNFRSF17
• Synonyms: TNFRSF17; tumor necrosis factor receptor superfamily, member 17; BCMA; tumor necrosis factor receptor superfamily member 17; BCM; CD269; B-cell maturation factor; B cell maturation antigen; B-cell maturation protein
• Gene ID: 608
• mRNA Refseq: NM_001192
• Protein Refseq: NP_001183
• MIM: 109545
• UniProt ID: Q02223

Citation

Directed evolution of adenine base editors with increased activity and therapeutic application.

Journal: Nature biotechnology    PubMed ID: 32284586    Data: 2020/10/7

Authors: Nicole M Gaudelli, Dieter K Lam, Giuseppe Ciaramella

Article Snippet:Methodology Sample preparation To assess editing efficiency, one million cells were taken from culture five days post electroporation and stained with the primary anti-human antibodies listed in the section above.Methodology Sample preparation To assess editing efficiency, one million cells were taken from culture five days post electroporation and stained with the primary anti-human antibodies listed in the section above.. Cell surface detection of CAR molecules utilized a PE-tagged, recombinant TNFRSF17 (BCMA) protein (Creative BioMart).. One million cells were labelled with LIVE/DEAD? Fixable Near-IR Dead Cell Stain Kit (Molecular Probes) according to manufacturer’s instructions.One million cells were labelled with LIVE/DEAD? Fixable Near-IR Dead Cell Stain Kit (Molecular Probes) according to manufacturer’s instructions.