| Species : |
Mouse |
| Source : |
Mammalian Cells |
| Tag : |
His |
| Protein Length : |
34-685;39-369;370-685 a.a. |
| Description : |
Bmper, also known asbone morphogenetic proteinbinding endothelial cell precursorderivedregulator, is a secreted chordinlike protein that modulates the BMP signalingpathway. Mouse Bmper is synthesized as a 685 amino acid (aa) residueprecursor protein with a putative 39 aa signal peptide, five tandemchordinlike cysteinerich (CR) domains, a partial von Willebrand factor type Ddomain (vWD), and a carboxyl trypsin inhibitorlike cysteinerich domain (TIL).Secreted Bmper is reported to be proteolytically cleaved to generate twofragments that are disulfide linked. |
| Form : |
Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| N-terminalSequence : |
His, Ala39 &Pro370 |
| Molecular Weight : |
36-39kDa & 50-55 kDa |
| Activity : |
Measuredby its ability to inhibit rhBMP-4-induced alkaline phosphatase production byATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem.Biophys. Res. Commun. 315:272. The ED50 for this effect istypically 0.5-2 μg/mL in the presence of 75 ng/mL of rhBMP-4. |
| Endotoxin Level : |
<1.0 EUper 1 μg of the protein by the LAL method. |
| SDS-PAGE : |
36-39kDa, reducing conditions |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstituteat 250 μg/mL in sterile PBS that containing at least 0.1% human or bovineserum albumin. |
| Storage : |
Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution. |
