Active Recombinant Mouse Epiphycan, His-tagged
Cat.No. : | Epyc-467M |
Product Overview : | Recombinant Mouse Epycprotein was expressed in Murine myeloma cell line with a C-terminal 10-His tag.Ala20-Ile322. |
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Cat. No. : | Epyc-467M |
Description : | Epyc, also called epiphycanor PGLb, is a 95-100 kDa class III subfamily member of the SLRP (small leucinerichproteoglycans) family of molecules. It is synthesized as a 322 amino acid (aa)precursor that contains a 19 aa signal sequence and a 303 aa mature region. Themature molecule contains three N-terminal O-glycosylation sites (including onefound to bind dermatin sulfate) and a glutamic acidrich motif followed by sixleucinerich repeats (LRR) that contain two potential Nglycosylation sites. |
Source : | Mammalian cells |
Form : | Lyophilized from a 0.2μm filtered solution in PBS. |
N-terminalSequence : | Ala20 |
Molecular Weight : | 36.2 kDa |
Activity : | Measuredby the ability of the immobilized protein to inhibit the adhesion of SAOS2 humanosteosarcoma cells (ATCC: HTB85) to human Fibronectin. When 5 x 104cells/well are added to Mouse Epyc and Human Fibronectin(0.5 μg/mL) coated plates, the cell adhesion is enhanced in a dose dependent manner.The ED50 for this effect is typically 0.3-1.2 μg/mL. Optimal dilutionsshould be determined by each laboratory for each application. |
Endotoxin Level : | <1.0 EUper 1 μg of the protein by the LAL method. |
Purity : | >95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution : | Reconstituteat 200 μg/mL in PBS |
Storage : | Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution. |
Protein length : | Ala20-Ile322 |
Gene Name : | Epyc epiphycan[ Mus musculus ] |
Official Symbol : | Epyc |
Synonyms : | Epyc; epiphycan;Dspg3; PG-Lb; SLRR3B; proteoglycan-Lb; dermatan sulfate proteoglycan 3; dermatansulphate proteoglycan 3; small chondroitin/dermatan sulfate proteoglycan; Pglb |
Gene ID : | 13516 |
mRNA Refseq : | NM_007884 |
Protein Refseq : | NP_031910 |
UniProt ID : | P70186 |
Chromosome Location : | 10 C2-C3; 10 55.0 cM |
Products Types
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EPYC-24H | Active Recombinant Human EPYC Protein (20-322aa), C-His tagged | +Inquiry |
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EPYC-3435H | Recombinant Human EPYC Protein, GST-tagged | +Inquiry |
◆ Lysates | ||
EPYC-6572HCL | Recombinant Human EPYC 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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