Species : |
Mouse |
Source : |
E.coli |
Tag : |
Non |
Description : |
IFN‐α2 is a type I interferon, previously known as B‐cell interferon, leukocyte interferon, lymphoblast interferon, and pH2‐stable interferon. IFN‐α2 is one of at least 23 different known variants of IFN‐α. The individual proteins have molecular masses between 19‐26 kDa and consist of proteins with lengths of 156‐166 and 172 amino acids. IFN‐α forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low‐molecular weight substances (n‐butyrate, 5‐bromodeoxyuridine). IFN‐α2 demonstrates antiviral, antiparasitic, antiproliferative activities. |
Molecular Mass : |
The protein has a predicted molecular mass of 19,361. The non‐reduced protein migrates as a 16 kDa polypeptide on SDS‐PAGE. The DTT reduced protein migrates as an 18 kDa polypeptide. |
Purity : |
Greater than 98% as determined by SDS‐PAGE. |
Form : |
Sterile liquid; 20 mM sodium phosphate, pH 7.2, 150 mM NaCl, with 0.5% BSA. 0.22 μM filtered. |
Endotoxin Level : |
Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
Bioactivity : |
The recombinant mouse IFN‐a2 has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED50 for this effect is typically 0.1 ng/ml, corresponding to a specific activity of 1 x 107 U/mg. |
Applications Tested : |
The recombinant mouse IFN‐α2 has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED50 for this effect is typically 0.1 ng/ml, corresponding to a specific activity of 1 x 107 U/mg. |
Temperature Limitation : |
Store at less than or equal to‐70°C. |
Handling Conditions : |
For best recovery, quick‐spin vial prior to opening. Use in a sterile environment. |