| Cat. No. : |
Il2-465M |
| Description : |
Mouse Interleukin-2, also know as T-cell Growth Factor, is an immunomodulatory cytokine produced by stimulated T-cells. Its activity is signaled via interaction with the IL-2R receptor. |
| Species : |
Mouse |
| Source : |
E.coli |
| Form : |
The protein (1mg/ml) was lyophilized after extensive dialysis against 10mM sodium citrate buffer to a pH=4. |
| Purity : |
Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Bio-activity : |
Recombinant mouse IL-2 is fully biologically active as compared to a standard. The ED50, determined by the dose dependent proliferation of mouse CTLL-2 cells, is 0.18ng/ml. |
| Physical Appearance : |
Sterile Filtered white lyophilized (freeze-dried) powder. |
| Solubility : |
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions. |
| Purity Greater than 98% as determined by : |
Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Protein Content determined by : |
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
| Endotoxin Level : |
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
| Amino acid sequence : |
PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ |
| Storage : |
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
| Pathways : |
Allograft rejection; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Graft-versus-host disease; Jak-STAT signaling pathway; T cell receptor signaling pathway; Type I diabetes mellitus |
