Active Recombinant Mouse Kitlg, His-tagged

• Cat.No.: Kitlg-34M
• Product Overview: Recombinant Mouse Kitlg was produced in the endosperm tissue of barley grain (Hordeum vulgare).

Specification

• Species: Mouse
• Source: Barley Grain
• Tag: His
• Description: Stem Cell Factor (SCF, also known as c-kit ligand, mast cell growth factor and steel factor) is a hematopoietic growth factor acting at the early stages of hematopoiesis. SCF promotes the survival, proliferation and differentiation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also stimulates the growth and activation of mast cells and eosinophils. SCF has been shown to act synergistically with other growth factors, such as GM-CSF, G-CSF, IL-7 and EPO. Human SCF shows low activity on murine cells, while murine and rat SCF are fully active on human cells.
• Form: Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2
• Bio-activity: Bioactivity of mouse SCF is measured by its dose-dependent effect on the proliferation of TF-1 cells. The ED50 for this effect is typically< 12="" ng/ml="" corresponding="" to="" specific="" activity="">8 x 10e4 U/mg. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay.
• Molecular Mass: Recombinant mouse SCF contains 166 amino acids and a 16 a.a. histidine-based tag for a total length of 182 a.a. and has a predicted molecular mass of 20.6 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 24 kDa in SDS-PAGE.
• Endotoxin: Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg).
• Purity: Greater than 95% by SDS-PAGE gel analysis
• Storage: The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Gene Information

• Gene Name: KITLG KIT ligand [ Homo sapiens ]
• Official Symbol: Kitlg
• Synonyms: KITLG; KIT ligand; MGF; kit ligand; familial progressive hyperpigmentation 2; FPH2; Kitl; KL 1; mast cell growth factor; SCF; SF; steel factor; stem cell factor; c-Kit ligand; KL-1; SHEP7; kit-ligand; DKFZp686F2250
• Gene ID: 4254
• mRNA Refseq: NM_000899
• Protein Refseq: NP_000890
• MIM: 184745
• UniProt ID: P21583
• Chromosome Location: 12q22
• Pathway: C-MYB transcription factor network, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Kit Receptor Signaling Pathway, organism-specific biosystem; Melanogenesis, organism-specific biosystem
• Function: cytokine activity; growth factor activity; protein binding; stem cell factor receptor binding