| Description : |
Oncostatin-M, also known as OSM, is a growth regulator belonging to the interleukin-6 group of cytokines. It is expressed mainly by monocytes, activated T cells and Kaposi’s sarcoma cells. OSM signals through two types of receptors; one is composed of gp130 and LIFR, and the other is composed of gp130 and OSMR. OSM regulates IL-6, G-CSF and GM-CSF production, and thus is involved in hematopoiesis, neurogenesis and osteogenesis. OSM displays both stimulatory and inhibitory effects, so its categorization as pro-inflammatory or anti-inflammatory cytokine is still controversial. |
| Source : |
HEK 293 |
| Species : |
Mouse |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 0.4 ng/mL, measured in a cell proliferation assay using NIH-3T3 cells. |
| Molecular Mass : |
10-40 kDa, observed by reducing SDS-PAGE. |
| AA Sequence : |
ANRGCSNSSSQLLSQLQNQANLTGN TESLLEPYIRLQNLNTPDLRAACTQ HSVAFPSEDTLRQLSKPHFLSTVYT TLDRVLYQLDALRQKFLKTPAFPKL DSARHNILGIRNNVFCMARLLNHSL EIPEPTQTDSGASRSTTTPDVFNTK IGSCGFLWGYHRFMGSVGRVFREWD DGSTRSRR |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE and HPLC. |
| Storage : |
Lyophilized recombinant Murine Oncostatin-M remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Murine Oncostatin-M should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
