Active Recombinant TEV Protease Protein (231 aa), N-His-tagged

• Cat.No.: Protease-308P
• Product Overview: Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged was expressed in E. coli. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques.

Specification

• Species: TEV
• Source: E.coli
• Tag: His
• Protein Length: 231
• Description: Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30 centigrade; also it can be used at temperature as low as 4 centigrade. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: 6 IU/μL
• Molecular Mass: 28.4 kDa, observed by reducing SDS-PAGE.
• Purity: > 95% by SDS-PAGE analyses.
• Unit Definition: One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30 centigrade.
• Storage: Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20 centigrade from date of receipt. Please avoid freeze-thaw cycles.
• Storage Buffer: Sterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH7.5.

Gene Information

• Official Symbol: Protease
• Synonyms: Protease