Tag : |
Non |
Size : |
100 assays |
Description : |
Angiotensin II converting enzyme (ACE2, EC 3.4.17.23), a carboxypeptidase, is part of the renin-angiotensin system (RAS) that controls regulation of blood pressure by cleaving the C-terminal dipeptide of Angiotensin II to convert it into Angiotensin 1-7. It also cleaves Angiotensin I to produce Ang 1-9, of unknown function. ACE2 is a receptor of human coronaviruses, such as SARS and HCoV-NL63. It is expressed on the vascular endothelial cells of heart and kidney. The inhibitors of ACE2 could be able to regulate hypertension by changing vascular permeability. Screening for small molecule and peptide inhibitors might also help in finding treatment for coronavirus mediated infection. |
Applications : |
Screening/characterizing/studying ACE2 inhibitors/activators |
Storage : |
Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.ACE2 Assay Buffer and ACE2 Dilution Buffer: Store at -20 °C or 4 °C. Bring to room temperature before use.ACE2 Enzyme: Store at -20°C. Thaw before use. Avoid multiple freeze/thaw of the enzyme. Use within 3 months.ACE2 Substrate: Ready to use. Store at -20°C. Thaw before use.ACE2 Inhibitor: Store at -20°C. Thaw before use. Avoid multiple freeze/thaw of the inhibitor. |
Kit Components : |
ACE2 Assay Buffer: 25 mlACE2 Dilution Buffer: 1.5 mlACE2 Enzyme: 20 µlACE2 Substrate: 200 µlACE2 Inhibitor (0.5 mM): 5 µl |
Detection method : |
Fluorescence (Ex/Em = 320/420 nm) |
Features & Benefits : |
• Simple and reliable test to screen ACE2 inhibitors/activators• High-throughput compatible• Includes Inhibitor Control |