ATAD2A Inhibitor Screening Assay Kit
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|Size :||384 reactions|
|Description :||The ATAD2A Inhibitor Screening Assay Kit is designed to measure the inhibition of ATAD2A binding to its substrate. The key to the ATAD2A Inhibitor Screening Assay Kit is a specific binding of the ATAD2A bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing ATAD2A and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.|
|Applications :||Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.|
|Storage :||At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.|
|Kit Components :||GST-ATAD2A: 100 µg; -80°CBET Bromodomain Ligand: 400 µl; -80°C Non-acetylated Ligand 1: 200 µl; -80°C 3x BRD Homogeneous Assay Buffer 2: 4 ml; -20°C3x BRD Homogeneous Detection Buffer 2: 3 ml; -20°C|