Cathepsin L Activity Fluorometric Assay Kit

• Cat.No.: Kit-0186
• Product Overview: Cathepsin L Activity Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme in biological samples or in purified enzyme preparations. The long wavelength FRET substrate is designed based on a sequence surrounding the cleavage site of Cathepsin L. A Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin L cleaves the FRET substrate, it results in an increase of Fluor 488 fluorescence and can be monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from Fluor 488 is stable at low pH, the optimal pH for Cathepsin L activity.

Specification

• Materials Required but Not Supplied: 96-well microplate: Black, flat bottom 96-well plate with non-binding surface. Fluorescence microplate reader: Capable of detecting emission at 520 nm with excitation at 490 nm.
• Kit Components: Component A: Cathepsin L substrate, Ex/Em=490 nm/520 nm upon cleavage: 1 mM, 50 µL Component B: Fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: Cathepsin L, human recombinant: 0.1 mg/mL, 20 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin L inhibitor: 100 µM, 10 µL Component F: DTT: 1 M, 200 µL
• Storage: Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
• Size: 100 Assays