Recombinant Human DDIT4 protein, His-tagged DDIT4-3433H

Cat.No.:	DDIT4-3433H
Product Overview:	Recombinant Human DDIT4(1-232aa) fused with His tag at N-terminal was expressed in E. coli.
Description:	DDIT4, also known as Dig2 or REDD1, is thought to have function in the regulation of reactive oxygen species. In response to stress due to DNA damage and glucocorticoid treatment, DDIT4 is upregulated at the transcriptional level. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation.
Source:	E. coli
Species:	Human
Tag:	His
Form:	Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol
Molecular Mass:	27.5 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
AA Sequence:	MGSSHHHHHH SSGLVPRGSHAYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT
Purity:	>90% by SDS - PAGE
Storage:	Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration:	0.25 mg/ml (determined by Bradford assay)

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Gene Name:	DDIT4 DNA-damage-inducible transcript 4 [ Homo sapiens ]
Official Symbol:	DDIT4
Synonyms:	DDIT4; DNA-damage-inducible transcript 4; DNA damage-inducible transcript 4 protein; Dig2; FLJ20500; HIF 1 responsive RTP801; REDD 1; REDD1; RTP801; HIF-1 responsive protein RTP801; HIF-1 responsive RTP801 (RTP801); protein regulated in development and DNA damage response 1; REDD-1; RP11-442H21.1;
Gene ID:	54541
mRNA Refseq:	NM_019058
Protein Refseq:	NP_061931
MIM:	607729
UniProt ID:	Q9NX09
Chromosome Location:	10q22.1
Pathway:	Direct p53 effectors, organism-specific biosystem; mTOR signaling pathway, organism-specific biosystem; mTOR signaling pathway, organism-specific biosystem; mTOR signaling pathway, conserved biosystem;
Function:	14-3-3 protein binding;