Recombinant Full Length Human DDIT4 protein, His-tagged

• Cat.No.: DDIT4-3433H
• Product Overview: Recombinant Human DDIT4(1-232aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-232 a.a.
• Description: DDIT4, also known as Dig2 or REDD1, is thought to have function in the regulation of reactive oxygen species. In response to stress due to DNA damage and glucocorticoid treatment, DDIT4 is upregulated at the transcriptional level. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol
• Molecular Mass: 27.5 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSHAYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: DDIT4 DNA-damage-inducible transcript 4 [ Homo sapiens ]
• Official Symbol: DDIT4
• Synonyms: DDIT4; DNA-damage-inducible transcript 4; DNA damage-inducible transcript 4 protein; Dig2; FLJ20500; HIF 1 responsive RTP801; REDD 1; REDD1; RTP801; HIF-1 responsive protein RTP801; HIF-1 responsive RTP801 (RTP801); protein regulated in development and DNA damage response 1; REDD-1; RP11-442H21.1
• Gene ID: 54541
• mRNA Refseq: NM_019058
• Protein Refseq: NP_061931
• MIM: 607729
• UniProt ID: Q9NX09
• Chromosome Location: 10q22.1
• Pathway: Direct p53 effectors, organism-specific biosystem; mTOR signaling pathway, organism-specific biosystem; mTOR signaling pathway, organism-specific biosystem; mTOR signaling pathway, conserved biosystem
• Function: 14-3-3 protein binding