Cat. No.: |
IL1B-559R |
Product Overview: |
RecombinantRhesus Macaque IL1B is a single non-glycosylated polypeptide chain containing153 amino acids. |
Description: |
IL1B is aproinflammatory cytokine produced in a variety of cells including monocytes,tissue macrophages, keratinocytes and other epithelial cells. IL1B bind tothe same receptor and have similar if not identical biological properties.These cytokines have a broad range of activities including, stimulation of thymocyteproliferation by inducing IL-2 release, B-cell maturation and proliferation, mitogenicFGF-like activity, and the ability to stimulate the release of prostaglandinand collagenase from synovial cells. |
Source: |
Rhesus Macaque |
Host: |
E. coli |
Form: |
Lyophilized from a 0.2 um sterile filteredsolution of PBS, pH 7.4. |
Purity: |
>98%by SDS-PAGE and HPLC analyses. |
Sequence: |
APVRSLHCTLRDAQLKSLVM SGPYELKALH LQGQDLEQQV VFSMSFVQGE ESNDKIPVAL GLKAKNLYLS CVLKDDKPTLQLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTRGG QDITDFTMQFVSS |
Molecular Weight: |
17.3 kDa |
Biological Activity: |
Fullybiologically active when compared to standard. The ED50 as determined by thedose-dependent stimulation of D10.G4.1 mouse helper T cells is typically 3-10pg/ml. |
Endotoxin: |
< 0.1 ng/μg ofprotein. |
Reconstitution: |
Centrifugevial prior to opening. Reconstitute in sterile distilled water or aqueousbuffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stocksolutions should be apportioned into working aliquots and stored at <-20°C.Further dilutions should be made in appropriate buffered solutions. |
Storage: |
Thelyophilized protein is stable at 2-4°C, but should be kept desiccated at-20°C for long term storage. After reconstitution, the protein is stable for1 week at 2-4°C. For long term storage, aliquot and freeze at -80°C. Avoidrepeated freeze-thaw cycles. |
OfficialSymbol: |
IL1B |
Pathways: |
Cytokine-cytokinereceptor interaction; Hematopoietic cell lineage; Africantrypanosomiasis; Alzheimer"s disease; Amoebiasis; Apoptosis; Chagas disease(American trypanosomiasis); Cytosolic DNA-sensing pathway; Graft-versus-hostdisease; Herpes simplex infection; Influenza A |