Cat. No. : |
VEGFA-715H |
Product Overview : |
Recombinant humanVascular Endothelial Growth Factor 165, a 23 kDa protein consisting of 165amino acid residues, is produced as a homodimer. |
Description : |
This gene is amember of the platelet-derived growth factor (PDGF)/vascular endothelialgrowth factor (VEGF) family and encodes a protein that is often found as adisulfide linked homodimer. This protein is a glycosylated mitogen thatspecifically acts on endothelial cells and has various effects. |
Source : |
Insect cells |
Species : |
Human |
Form : |
Lyophilized withoutadditives. |
Purity : |
> 90% asdetermined by SDS-PAGE and visualized by silver stain. |
Molecular Mass : |
45 kDa |
Endotoxin Level : |
< 0.1 ng/μg ofVEGF165. |
Biological Activity : |
Determined by thestimulation of 3H-thymidine incorporation and cell proliferationby human umbilical vein endothelial cells. The ED50 range is 1-2ng/ml. |
Reconstitution : |
Centrifuge vialprior to opening. Add PBS or medium containing at least 0.1% HSA or BSA tothe vial to fully solubilize the protein to a concentration of 50 μg/ml.Please note that the addition of any carrier protein into this product mayproduce unwanted endotoxin. This depends upon the particular application employed. |
Storage : |
Lyophilized proteinis stable for 6 months at -20°C to -80°C. Store reconstituted protein inworking aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
OfficialSymbol : |
VEGFA |
Pathways : |
Bladder cancer;Cytokine-cytokine receptor interaction; Endochondral Ossification; Focaladhesion; Glypican 1 network; HIF-1-alpha transcription factor network;HIF-2-alpha transcription factor network; Heart Development; Hemostasis; HypertrophyModel; Id Signaling Pathway; Integrins in angiogenesis; Neurophilininteractions with VEGF and VEGFR; Pancreatic cancer; Pathways in cancer |