||Recombinant humanANAPC13 protein fused to T7-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||ANAPC13,also known as anaphase-promoting complex subunit 13, is component of theanaphase promoting complex, a large ubiquitin-protein ligase that controlscell cycle progression by regulating the degradation of cell cycle regulatorssuch as B-type cyclins.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl.
||10.0 kDa(89aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appearhigher)
||> 90%by SDS-PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MASMTGGQQMGRGSHMDSEV QRDGRILDLI DDAWREDKLP YEDVAIPLNE LPEPEQDNGG TTESVKEQEM KWTDLALQYLHENVPPIGN
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||FOXA2 and FOXA3 transcription factornetworks; Fructose and mannose metabolism; Fructose catabolism;Gluconeogenesis; Glucose metabolism; Glycolysis; Glycolysis (Embden-Meyerhofpathway); Glycolysis / Gluconeogenesis; Glycolysis and Gluconeogenesis;Metabolic pathways; Metabolism of carbohydrates; Pentose phosphate pathway