| Cat. No. : |
Agrp-546M |
| Description : |
Agrp, the proteinproduct of the Agouti Related Transcript (ART), is a neuroprotein thatregulates energy metabolism and the development of obesity by antagonizing αmelanocytestimulating hormone (α-MSH) action on MC-3 and MC-4 receptors. Agrp is predominantlyexpressed in the hypothalamus and adrenal medulla. Mature mouse Agrp is a 111amino acid (aa) polypeptide; its C-terminal portion contains ten conservedcysteines that form five disulfide bonds. Within the C-terminal region, mouseAgrp shares 80% and 90% aa sequence identity with human and rat Agrp,respectively. It also shares 44% aa sequence identity with Agrp. As with Agrp,the C-terminal cysteine rich region is sufficient for biological activity. |
| Source : |
E. coli-derived |
| Form : |
Lyophilized from a0.2 μm filtered solution in PBS. |
| N-terminalSequence : |
Ser82 |
| Molecular Weight : |
5.7 kDa |
| Activity : |
Measured by itsability to antagonize α-MSHinduced c-AMP accumulation on human MelanocortinReceptor 4 transfected BaF3 mouse proB cells. The ED50 for thiseffect is typically 1-4 μg/mL in the presence of 10 ng/mL of α-MSH. |
| Endotoxin Level : |
< 1.0EU per 1 μg of the protein by the LAL method. |
| Purity : |
> 95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
100μg/mL in sterile PBS. |
| Storage : |
Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. |
