Recombinant Mouse Dickkopf Homolog 4 (Xenopus laevis), His-tagged Dkk4-404M

Cat. No.:	Dkk4-404M
Product Overview:	Recombinantmouse Dkk4 protein was expressed in Murine myeloma cell line, NS0-derived, with a C-terminal6-His tag.Leu19-Ile221.
Description:	Dkk4 is a member ofthe Dickkopf protein family that includes Dkk -1, -2, -3, and -4 and arelated protein, Soggy. Expression of Dkk4 has only been shown with sensitivePCR techniques during early embryonic development in mice, in differentiatedhuman ES cells, or in mice that express dominant-active β-catenin elevatingWnt signaling in the forebrain.
Source:	Murine myeloma cellline, NS0-derived
Form:	Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
N-terminal Sequence:	Leu19
Molecular Weight:	23 kDa
Activity:	Measured by itsability to inhibit proliferation in MC3T3-E1 mouse osteoblastic cells. The ED50for this effect is typically 1-4 μg/mL. Optimal concentrations should bedetermined by each laboratory for each application.
Endotoxin Level:	<1.0 EU per 1 μgof the protein by the LAL method.
Purity:	>95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution:	Reconstitute at 100μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
Storage:	Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

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Gene Name:	Dkk4dickkopf homolog 4 (Xenopus laevis) [ Mus musculus ]
Official Symbol:	Dkk4
Synonyms:	Dkk4;dickkopf homolog 4 (Xenopus laevis); Dkk-4; MGC25705; dickkopf-relatedprotein 4; dickkopf-4
Gene ID:	234130
mRNA Refseq:	NM_145592
Protein Refseq:	NP_663567
UniProt ID:	Q8VEJ3
Chromosome Location:	8
Pathway:	Wnt signalingpathway