"Dkk4" Related Products

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Recombinant Mouse Dickkopf Homolog 4 (Xenopus laevis), His-tagged

Cat. No.: Dkk4-404M
Product Overview: Recombinantmouse Dkk4 protein was expressed in Murine myeloma cell line, NS0-derived, with a C-terminal6-His tag.Leu19-Ile221.
Description: Dkk4 is a member ofthe Dickkopf protein family that includes Dkk -1, -2, -3, and -4 and arelated protein, Soggy. Expression of Dkk4 has only been shown with sensitivePCR techniques during early embryonic development in mice, in differentiatedhuman ES cells, or in mice that express dominant-active β-catenin elevatingWnt signaling in the forebrain.
Source: Murine myeloma cellline, NS0-derived
Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
N-terminal Sequence: Leu19
Molecular Weight: 23 kDa
Activity: Measured by itsability to inhibit proliferation in MC3T3-E1 mouse osteoblastic cells. The ED50for this effect is typically 1-4 μg/mL. Optimal concentrations should bedetermined by each laboratory for each application.
Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstitute at 100μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.
Gene Name: Dkk4dickkopf homolog 4 (Xenopus laevis) [ Mus musculus ]
Official Symbol: Dkk4
Synonyms: Dkk4;dickkopf homolog 4 (Xenopus laevis); Dkk-4; MGC25705; dickkopf-relatedprotein 4; dickkopf-4
Gene ID: 234130
mRNA Refseq: NM_145592
Protein Refseq: NP_663567
UniProt ID: Q8VEJ3
Chromosome Location: 8
Pathway: Wnt signalingpathway

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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