||Recombinant humanSULT1B1 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.
||Sulfotransferasefamily, cytosolic, 1B, member 1, also known as SULT1B1, is an enzyme that inhumans is encoded by the SULT1B1 gene. SULT1B1 contains a binding site forthe sulfate donor, 3-prime-phosphoadenosine 5-prime-phosphosulfate, and acysteine residue conserved in the ST1 gene family of sulfotransferases.Sulfotransferases such as SULT1B1 catalyze the biotransformation of a largenumber of endogenous compounds such as neurotransmitters, steroids, bileacids, and thyroid hormones, as well as drugs and xenobiotics.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
||37.4kDa (320aa),confirmed by MALDI-TOF
||> 95% by SDS -PAGE
||1 mg/ml (determinedby Bradford
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMLSPKD ILRKDLKLVH GYPMTCAFAS NWEKIEQFHS RPDDIVIATY PKSGTTWVSEIIDMILNDGD IEKCKRGFIT EKVPMLEMTL PGLRTSGIEQ LEKNPSPRIV KTHLPTDLLP KSFWENNCKMIYLARNAKDV SVSYYHFDLM NNLQPFPGTW EEYLEKFLTG KVAYGSWFTH VKNWWKKKEE HPILFLYYEDMKENPKEEIK KIIRFLEKNL NDEILDRIIH HTSFEVMKDN PLVNYTHLPT TVMDHSKSPF MRKGTAGDWKNYFTVAQNEK FDAIYETEMS KTALQFRTEI
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°Cor -70°C. Avoid repeated freezing and thawing cycles.