Expand
Expand
| Cat.No.: | CAB11495RH |
| Product Overview: | Rabbit monoclonal antibody to human CD27 molecule, FITC-conjugated. |
| Antigen Description: | CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, and during which, adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling processes. In addition, a proapoptotic protein named SIVA, is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis. |
| Specificity: | Human CD27 / TNFRSF7, Has cross-reactivity in ELISA with, Mouse CD27, No cross-reactivity in ELISA with, Human TNFRSF8/CD30, Human 4-1BB, Human CD40, Human DR6, Human FAS, Human HVEM/TNFRSF14, Human TNFRI, Human TNFRII, Human BAFF, Human RELT, Mouse HVEM, Mouse FAS,Rat FAS. |
| Immunogen: | Recombinant human CD27 / TNFRSF7 protein |
| Host: | Rabbit |
| Isotype: | Rabbit IgG |
| Species: | Human |
| Clone: | 024 |
| Conjugation: | FITC |
| Applications: | Western Blot; Flow Cytometry |
| Dilution: | Flow Cytometry: Human lymphocytes were stained with anti human CD27-CAB11512 (solid line) or rabbit IgG isotype control (dashed line).Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human CD27 in WB. Use of this antibody under reducing conditions is not recommended. |
| Preparation: | This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CD27 and conjugated with FITC under optimum conditions, the unreacted FITC was removed. |
| Format: | Aqueous solution containing 0.5% BSA and 0.1% sodium azide |
| Concentration: | 5 μl/Test, 0.2 mg/ml |
| Storage: | This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze!Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Gene Name: | CD27 CD27 molecule [ Homo sapiens ] |
| Official Symbol: | CD27 |
| Synonyms: | CD27; CD27 molecule; TNFRSF7,tumor necrosis factor receptor superfamily, member 7; CD27 antigen; S152; Tp55; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; T14; TNFRSF7; MGC20393; |
| Gene ID: | 939 |
| mRNA Refseq: | NM_001242 |
| Protein Refseq: | NP_001233 |
| MIM: | 186711 |
| UniProt ID: | P26842 |
| Chromosome Location: | 12p13 |
| Pathway: | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; |
| Function: | cysteine-type endopeptidase inhibitor activity involved in apoptotic process; protein binding; receptor activity; transmembrane signaling receptor activity; |
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Download Datasheet
See All CD27 Products
Download Datasheet