Recombinant Human PFDN5 protein, His-tagged

• Cat.No.: PFDN5-3608H
• Product Overview: Recombinant Human PFDN5(1-154aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: PFDN5 belongs to the prefoldin alpha subunit family. Prefoldin (PFDN), a ubiquitously expressed heterohexameric co-chaperone, is necessary for proper folding of nascent proteins, in particular, tubulin and actin. PFDN5 is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. This protein may also repress the transcriptional activity of the proto-oncogene c-Myc.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol
• Molecular Mass: 19.5kDa (174aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSHYVPGKLHDVE HVLIDVGTGY YVEKTAEDAK DFFKRKIDFL TKQMEKIQPA LQEKHAMKQA VMEMMSQKIQ QLTALGAAQA TAKAMAQSINITEL NLPQLEMLKN QLDQEVEFLS TSIAQLKVVQ TKYVEAKDCL NVLNKSNEGK ELLVPLTSSM
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)
• Publication:
  Prefoldin 5 and anti-prefoldin 5 antibodies as biomarkers for uveitis in ankylosing spondylitis (2019)

Gene Information

• Gene Name: PFDN5 prefoldin subunit 5 [ Homo sapiens ]
• Official Symbol: PFDN5
• Synonyms: PFDN5; prefoldin subunit 5; prefoldin 5; MM 1; PFD5; myc modulator-1; c-myc binding protein; MM1; MM-1; MGC5329; MGC71907
• Gene ID: 5204
• mRNA Refseq: NM_002624
• Protein Refseq: NP_002615
• MIM: 604899
• UniProt ID: Q99471
• Chromosome Location: 12q13.13
• Pathway: Chaperonin-mediated protein folding, organism-specific biosystem; Cooperation of Prefoldin and TriC/CCTin actin and tubulin folding, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; Prefoldin mediated transfer of substrateto CCT/TriC, organism-specific biosystem; Protein folding, organism-specific biosystem
• Function: protein binding; transcription corepressor activity; unfolded protein binding