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Recombinant Mouse Carbonic Anhydrase 2, His-tagged

Cat.No. : Car2-1191M
Product Overview : A DNA sequence encoding the mouse Car2 ( AAH55291.1 ) ( Met 1 ?C Lys 260 ) was expressed, with a C-terminal polyhistidine tag
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Cat. No. : Car2-1191M
Description : Carbonic anhydrases ( Cas ) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature.
Source : Mouse
Host : E.coli
Form : Lyophilized from sterile 50 mM Tris, 150mM NaCl , pH 7.5 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Purity : > 96 % as determined by SDS-PAGE
Endotoxin : μ1.0 EU per μg protein as determined by the LAL method
Stability : Samples are stable for up to twelve months from date of receipt at -70 μ
Predicted N terminal : Ser 2
Molecular Mass : The recombinant mouse Car2 comprises 270 amino acids and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions.
Storage : Store it under sterile conditions at -70μ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Pathways : Bile secretion; Collecting duct acid secretion; Gastric acid secretion; Nitrogen metabolism; Pancreatic secretion
Gene Name : Car2 carbonic anhydrase 2 [ Mus musculus ]
Official Symbol : Car2
Synonyms : Car2; Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712; 3 A1; 3 10.5 Cm; carbonic anhydrase 2; CA II; CA-II; carbonic anhydrase II; carbonate dehydratase II
Gene ID : 12349
mRNA Refseq : NM_009801
Protein Refseq : NP_033931
UniProt ID : P00920
Chromosome Location : 3 A1; 3 10.5 cM
Function : carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Is CA2 involved in any particular pathological conditions? 07/05/2022

CA2 deficiency is a rare autosomal recessive disorder with metabolic acidosis and osteopetrosis.

What are the known inhibitors of CA2 and their clinical implications? 02/23/2022

Acetazolamide is a known inhibitor, used clinically for glaucoma and altitude sickness.

How do mutations in the CA2 gene influence enzyme activity or stability? 06/13/2021

Some mutations can lead to CA2 deficiency, affecting acid-base balance and leading to metabolic acidosis.

How does CA2's enzymatic activity compare to other carbonic anhydrase isoforms? 07/28/2019

CA2 is one of the most efficient and fastest enzymes, displaying higher activity than many other isoforms.

What role does CA2 play in the physiological regulation of pH? 07/23/2019

CA2 accelerates the reversible conversion of CO2 and water to bicarbonate and protons, aiding in pH regulation.

Are there specific cellular pathways where CA2 plays a significant role? 11/14/2018

CA2 plays a crucial role in CO2 transport and acid-base balance in various tissues.

How is CA2 expression distributed across different human tissues? 07/04/2018

CA2 is ubiquitously expressed but is particularly abundant in red blood cells and certain epithelial tissues.

Customer Reviews (3)

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Reviews
05/01/2022

    Efficient sample processing, a time-saving boon for researchers.

    08/30/2021

      Professional expertise, elevates our protein research projects.

      06/08/2021

        Invaluable assistance for intricate protein experiments.

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