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Recombinant Mouse Carbonic Anhydrase 2, His-tagged

Cat. No.: Car2-1191M
Product Overview: A DNA sequence encoding the mouse Car2 ( AAH55291.1 ) ( Met 1 ?C Lys 260 ) was expressed, with a C-terminal polyhistidine tag
Description: Carbonic anhydrases ( Cas ) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature.
Source: Mouse
Host: E.coli
Form: Lyophilized from sterile 50 mM Tris, 150mM NaCl , pH 7.5 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Purity: > 96 % as determined by SDS-PAGE
Endotoxin: μ1.0 EU per μg protein as determined by the LAL method
Stability: Samples are stable for up to twelve months from date of receipt at -70 μ
Predicted N terminal: Ser 2
Molecular Mass: The recombinant mouse Car2 comprises 270 amino acids and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions.
Storage: Store it under sterile conditions at -70μ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Pathways: Bile secretion; Collecting duct acid secretion; Gastric acid secretion; Nitrogen metabolism; Pancreatic secretion
Gene Name: Car2 carbonic anhydrase 2 [ Mus musculus ]
Official Symbol: Car2
Synonyms: Car2; Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712; 3 A1; 3 10.5 Cm; carbonic anhydrase 2; CA II; CA-II; carbonic anhydrase II; carbonate dehydratase II
Gene ID: 12349
mRNA Refseq: NM_009801
Protein Refseq: NP_033931
UniProt ID: P00920
Chromosome Location: 3 A1; 3 10.5 cM
Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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