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| Cat. No.: | Car2-1191M |
| Product Overview: | A DNA sequence encoding the mouse Car2 ( AAH55291.1 ) ( Met 1 ?C Lys 260 ) was expressed, with a C-terminal polyhistidine tag |
| Description: | Carbonic anhydrases ( Cas ) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. |
| Source: | Mouse |
| Host: | E.coli |
| Form: | Lyophilized from sterile 50 mM Tris, 150mM NaCl , pH 7.5 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Purity: | > 96 % as determined by SDS-PAGE |
| Endotoxin: | μ1.0 EU per μg protein as determined by the LAL method |
| Stability: | Samples are stable for up to twelve months from date of receipt at -70 μ |
| Predicted N terminal: | Ser 2 |
| Molecular Mass: | The recombinant mouse Car2 comprises 270 amino acids and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions. |
| Storage: | Store it under sterile conditions at -70μ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. |
| Pathways: | Bile secretion; Collecting duct acid secretion; Gastric acid secretion; Nitrogen metabolism; Pancreatic secretion |
| Gene Name: | Car2 carbonic anhydrase 2 [ Mus musculus ] |
| Official Symbol: | Car2 |
| Synonyms: | Car2; Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712; 3 A1; 3 10.5 Cm; carbonic anhydrase 2; CA II; CA-II; carbonic anhydrase II; carbonate dehydratase II |
| Gene ID: | 12349 |
| mRNA Refseq: | NM_009801 |
| Protein Refseq: | NP_033931 |
| UniProt ID: | P00920 |
| Chromosome Location: | 3 A1; 3 10.5 cM |
| Function: | carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding |
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