Cat. No.: |
Car2-1191M |
Product Overview: |
A DNA sequence encoding the mouse Car2 ( AAH55291.1 ) ( Met 1 ?C Lys 260 ) was expressed, with a C-terminal polyhistidine tag |
Description: |
Carbonic anhydrases ( Cas ) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. |
Source: |
Mouse |
Host: |
E.coli |
Form: |
Lyophilized from sterile 50 mM Tris, 150mM NaCl , pH 7.5 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Purity: |
> 96 % as determined by SDS-PAGE |
Endotoxin: |
μ1.0 EU per μg protein as determined by the LAL method |
Stability: |
Samples are stable for up to twelve months from date of receipt at -70 μ |
Predicted N terminal: |
Ser 2 |
Molecular Mass: |
The recombinant mouse Car2 comprises 270 amino acids and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions. |
Storage: |
Store it under sterile conditions at -70μ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. |
Pathways: |
Bile secretion; Collecting duct acid secretion; Gastric acid secretion; Nitrogen metabolism; Pancreatic secretion |