Active Recombinant Human CSF2 (18 - 144aa)

• Cat.No.: CSF2-025E
• Product Overview: Recombinant Human CSF2 (Ala18 ­ Glu144) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Specification

• Cat. No.: CSF2-025E
• Description: GM­CSF was initially characterized as a factor that granulocyte­macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM­CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM­CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils.
• Source: E. coli
• N­terminal Sequence Analysis: Ala18
• Molecular Mass: 14 kDa
• Activity: Measured in a cell proliferation assay using TF­1 human erythroleukemic cells. The ED50 for this effect is typically 6 ­ 30 pg/mL.
• Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
• Unitprot ID: P04141

Gene Information

• Gene Name: CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Homo sapiens ]
• Official Symbol: CSF2
• Synonyms: CSF2; colony stimulating factor 2 (granulocyte-macrophage); GMCSF; MGC131935; MGC138897; granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; colony-stimulating factor; granulocyte-macrophage colony stimulating factor
• Gene ID: 1437
• mRNA Refseq: NM_000758
• Protein Refseq: NP_000749
• MIM: 138960
• Chromosome Location: 5q31.1
• Pathway: Amoebiasis; Calcium signaling in the CD4+ TCR pathway; Cytokine Signaling in Immune system; Cytokine-cytokine receptor interaction; Cytokines and Inflammatory Response; Fc epsilon RI signaling pathway; Interleukin receptor SHC signaling; Jak-STAT signaling pathway; T cell receptor signaling pathway
• Function: cytokine activity; granulocyte colony-stimulating factor receptor binding; growth factor activity; protein binding