||HGF is a disulfide linked heterodimer with μ and μ chain. DTT-reduced protein migrates as 60-70 kDa and 30-40 kDa in SDS-PAGE. And in non-reducing conditions , the protein migrates in 65-75 kDa.
||Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells. It has been shown to have a major role in embryonic organ development, in adult organ regeneration and in wound healing. HGF was found to be identical to scatter factor, a fibroblast derived soluble factor that promotes the dissociation of epithelial and vascular endothelial cell colonies in monolayer cultures by stimulating cell migration.
||Recombinant human HGF Gln32-Ser728 (Accession P14210.2) was produced in human HEK293 cells.
||Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
||>95% as determined by SDS-PAGE. All lots are greater than 95% pure.
||Less than 1.0 EU per 1 μg of the HGF by the LAL method.
||Store at -80°C upon received. Avoid repeated freeze-thaw cycles.