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Recombinant Mouse Il1b (118-269aa), Met tagged

Cat. No.: Il1b-040E
Product Overview: Recombinant Mouse IL1B (Val118 ­ Ser269, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.
Description: IL­1 is a name that designates two pleiotropic cytokines, IL­1μ (IL­1F1) and IL­1μ (IL­1F2), which are the products of distinct genes. IL­1μ and IL­1μ are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL­1μ and same receptor and exert identical biological effects. IL­1 RI binds directly to IL­1μ or IL­1μ and then associates with IL­1 R accessory protein (IL­1 R3/IL­1 R AcP) to form a high­affinity receptor complex that is competent for signal transduction. IL­1 RII has high affinity for IL­1μ but functions as adecoy receptor and negative regulator of IL­1μ activity. IL­1ra functions as a competitive antagonist by preventing IL­1μ and IL­1μ from interacting with IL­1 RI. The human IL­1μ cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL­1μ­converting enzyme (Caspase­1/ICE) to generate the active cytokine.
Source: E. coli
N­terminal Sequence Analysis: Met
Molecular Mass: 17 kDa
Activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 5 ­ 10 pg/mL.
Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.
Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
Unitprot ID: P10749
Gene Name: Il1b interleukin 1 beta [ Mus musculus ]
Official Symbol: Il1b
Synonyms: Il1b; interleukin 1 beta; Il-1b; IL-1beta; interleukin-1 beta
Gene ID: 16176
mRNA Refseq: NM_008361
Protein Refseq: NP_032387
Chromosome Location: 2 F; 2 73.0 cM
Pathway: Alzheimer"s disease; Apoptosis; Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; MAPK signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus; negative regulation of insulin receptor signaling pathway
Function: cytokine activity; growth factor activity; interleukin-1 receptor binding; protein domain specific binding

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