Species : |
Human |
Source : |
Sf9 Cells |
Tag : |
GST |
Description : |
CSNK1G2 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CSNK1G2 is a ubiquitously expressed cytoplasmic kinase that can interact and phosphorylate the metastatic tumor antigen 1 short form (MTA1s) co-localizing it in the cytoplasm. Phosphorylated MTA1s then sequesters the estrogen receptor-alpha in the cytoplasm of breast cancer cells. CSNK1G2 hyperphosphorylates the ceramide transfer protein CERT leading to a decrease in de novo sphingomyelin synthesis. The reduction in synthesis of sphingomyelin is reversed by the expression of CERT mutants that are not hyperphosphorylated. |
Applications : |
Kinase Assay; Western Blot |
Molecular Weight : |
73 kDa |
Expression System : |
Sf9 insect cells using baculovirus |
Form : |
Recombinant protein stored in 50 mM Tris-HCl, ph 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. |
Specific Activity : |
30 nmol/min/mg |
Purity : |
> 80 % |
Concentration : |
0.1 ug/ul |
Sequences : |
Full length |
Storage : |
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Pathways : |
FoxO family signaling; Hedgehog signaling events mediated by Gli proteins; Hedgehog signaling pathway; Metabolism of lipids and lipoproteins; Sphingolipid metabolism; p53 pathway |
Full Length : |
Full L. |