| Cat.No. : | Vegf-1882M |
| Product Overview : | Recombinant mouse Vascular Endothelial Growth Factor A164 (rmVEGF-A164) produced in Pichia pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. |
| Description : | Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. |
| Source : | Pichia pastoris |
| Species : | Mouse |
| Form : | Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0. |
| Bio-activity : | Resuspend cells in assay media containing 10 % HI-FBS and transfer 100 ul/well to assay plate (5,100 cells/well). Serial dilute murine VEGF in assay media containing 10 % HI-FBS and transfer 100ml/well to cells in assay plate. Final assay volume is 200 ml/well, containing 10 % HI-FBS, and murine VEGF as indicated 89 hours. Add 20 ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well, Incubate 37°C and read at OD 490 nm. The ED50 for the effect is typically 4.1 - 6.2 ng/ml. |
| Molecular Mass : | 39kDa, observed by non-reducing SDS-PAGE. |
| AA Sequence : | Ala27-Arg190, expressed with an N-terminal Met. |
| Endotoxin : | <1.0 eu/μg, determined by lal> |
| Purity : | >97% as analyzed by reducing SDS-PAGE |
| Storage : | Lyophilized recombinant protein remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rmVEGF-A164 should be stable up to 4 week at 4°C or up to 6 months at -20°C. |
| Reconstitution : | Reconstituted in ddH2O at 100 μg/ml. |
| Gene Name : | Vegfa vascular endothelial growth factor A [ Mus musculus (house mouse) ] |
| Official Symbol : | Vegf |
| Synonyms : | Vegfa; Vpf; Vegf; Vegf120; Vegf164; Vegf188; vascular endothelial growth factor A; vascular permeability factor |
| Gene ID : | 22339 |
| UniProt ID : | Q00731 |
| Chromosome Location : | 17 C; 17 22.79 cM |
| Pathway : | Bladder cancer, organism-specific biosystem; Cellular response to hypoxia, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem |
| Function : | cell surface binding; chemoattractant activity; cytokine activity; cytokine activity |
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