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Active Recombinant Mouse Vegf

Cat.No. : Vegf-1882M
Product Overview : Recombinant mouse Vascular Endothelial Growth Factor A164 (rmVEGF-A164) produced in Pichia pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each.
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Description : Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.
Source : Pichia pastoris
Species : Mouse
Form : Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0.
Bio-activity : Resuspend cells in assay media containing 10 % HI-FBS and transfer 100 ul/well to assay plate (5,100 cells/well). Serial dilute murine VEGF in assay media containing 10 % HI-FBS and transfer 100ml/well to cells in assay plate. Final assay volume is 200 ml/well, containing 10 % HI-FBS, and murine VEGF as indicated 89 hours. Add 20 ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well, Incubate 37°C and read at OD 490 nm. The ED50 for the effect is typically 4.1 - 6.2 ng/ml.
Molecular Mass : 39kDa, observed by non-reducing SDS-PAGE.
AA Sequence : Ala27-Arg190, expressed with an N-terminal Met.
Endotoxin : <1.0 eu/μg, determined by lal>
Purity : >97% as analyzed by reducing SDS-PAGE
Storage : Lyophilized recombinant protein remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rmVEGF-A164 should be stable up to 4 week at 4°C or up to 6 months at -20°C.
Reconstitution : Reconstituted in ddH2O at 100 μg/ml.
Gene Name : Vegfa vascular endothelial growth factor A [ Mus musculus (house mouse) ]
Official Symbol : Vegf
Synonyms : Vegfa; Vpf; Vegf; Vegf120; Vegf164; Vegf188; vascular endothelial growth factor A; vascular permeability factor
Gene ID : 22339
UniProt ID : Q00731
Chromosome Location : 17 C; 17 22.79 cM
Pathway : Bladder cancer, organism-specific biosystem; Cellular response to hypoxia, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem
Function : cell surface binding; chemoattractant activity; cytokine activity; cytokine activity

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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