| Species : |
Mouse |
| Source : |
P.pastoris |
| Tag : |
Non |
| Description : |
Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. |
| Form : |
Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0. |
| Bio-activity : |
Resuspend cells in assay media containing 10 % HI-FBS and transfer 100 ul/well to assay plate (5,100 cells/well). Serial dilute murine VEGF in assay media containing 10 % HI-FBS and transfer 100ml/well to cells in assay plate. Final assay volume is 200 ml/well, containing 10 % HI-FBS, and murine VEGF as indicated 89 hours. Add 20 ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well, Incubate 37°C and read at OD 490 nm. The ED50 for the effect is typically 4.1 - 6.2 ng/ml. |
| Molecular Mass : |
39kDa, observed by non-reducing SDS-PAGE. |
| AA Sequence : |
Ala27-Arg190, expressed with an N-terminal Met. |
| Endotoxin : |
<1.0 eu/μg,="" determined="" by="" lal=""> |
| Purity : |
>97% as analyzed by reducing SDS-PAGE |
| Storage : |
Lyophilized recombinant protein remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rmVEGF-A164 should be stable up to 4 week at 4°C or up to 6 months at -20°C. |
| Reconstitution : |
Reconstituted in ddH2O at 100 μg/ml. |
