"NT5C3B" Related Products

Recombinant Human 5'-nucleotidase, cytosolic IIIB, His-tagged

Cat.No.: NT5C3B-664H
Product Overview: NT5C3B, 1-300aa Human, His tag, E.coli
Description: NT5C3B, also known 7-methylguanosine phosphate-specific 5"-nucleotidase, includes transferase activity and nucleotide binding. Among its related super-pathways are adenosine nucleotides degradation II and Pyrimidine metabolism. Specifically it hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also it has weak activity for CMP. Recombinant human NT5C3B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Source: E.coli
Species: Human
Tag: His-tag
Form: Liquid. Phosphate Buffered Saline (pH7.4) containing 20% glycerol, 1mM DTT
Molecular Mass: 36.8 kDa(323aa) confirmed by MALDI-TOF
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEEVST LMKATVLMRQ PGRVQEIVGA LRKGGGDRLQ VISDFDMTLS RFAYNGKRCP SSYNILDNSK IISEECRKEL TALLHHYYPI EIDPHRTVKE KLPHMVEWWT KAHNLLCQQK IQKFQIAQVV RESNAMLREG YKTFFNTLYH NNIPLFIFSA GIGDILEEII RQMKVFHPNI HIVSNYMDFN EDGFLQGFKG QLIHTYNKNS SACENSGYFQ QLEGKTNVIL LGDSIGDLTM ADGVPGVQNI LKIGFLNDKV EERRERYMDS YDIVLEKDET LDVVNGLLQH ILCQGVQLEM QGP
Purity: >85% by SDS - PAGE
Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration: 1 mg/ml (determined by Bradford assay)
Gene Name: NT5C3B 5"-nucleotidase, cytosolic IIIB [ Homo sapiens (human) ]
Official Symbol: NT5C3B
Synonyms: NT5C3B; NT5C3L; 5"-nucleotidase, cytosolic IIIB; 7-methylguanosine phosphate-specific 5"-nucleotidase; cN-III-like protein; cytosolic 5"-nucleotidase 3B; 7-methylguanosine nucleotidase; 5"-nucleotidase, cytosolic III-like; N(7)-methylguanylate 5"-phosphatase; cytosolic 5"-nucleotidase III-like protein; NP_443167.4; EC 3.1.3.5; EC 3.1.3.91
Gene ID: 115024
mRNA Refseq: NM_052935
Protein Refseq: NP_443167
UniProt ID: Q969T7
Chromosome Location: 17q21.2
Pathway: Nicotinate and nicotinamide metabolism; Purine metabolism; Pyrimidine metabolism
Function: 5"-nucleotidase activity; magnesium ion binding; nucleotide binding
Products Types ◆ Recombinant Protein

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