Recombinant Human 5'-nucleotidase, cytosolic IIIB, His-tagged

• Cat.No.: NT5C3B-664H
• Product Overview: NT5C3B, 1-300aa Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-300 a.a.
• Description: NT5C3B, also known 7-methylguanosine phosphate-specific 5"-nucleotidase, includes transferase activity and nucleotide binding. Among its related super-pathways are adenosine nucleotides degradation II and Pyrimidine metabolism. Specifically it hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also it has weak activity for CMP. Recombinant human NT5C3B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Form: Liquid. Phosphate Buffered Saline (pH7.4) containing 20% glycerol, 1mM DTT
• Molecular Mass: 36.8 kDa(323aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEEVST LMKATVLMRQ PGRVQEIVGA LRKGGGDRLQ VISDFDMTLS RFAYNGKRCP SSYNILDNSK IISEECRKEL TALLHHYYPI EIDPHRTVKE KLPHMVEWWT KAHNLLCQQK IQKFQIAQVV RESNAMLREG YKTFFNTLYH NNIPLFIFSA GIGDILEEII RQMKVFHPNI HIVSNYMDFN EDGFLQGFKG QLIHTYNKNS SACENSGYFQ QLEGKTNVIL LGDSIGDLTM ADGVPGVQNI LKIGFLNDKV EERRERYMDS YDIVLEKDET LDVVNGLLQH ILCQGVQLEM QGP
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NT5C3B 5"-nucleotidase, cytosolic IIIB [ Homo sapiens (human) ]
• Official Symbol: NT5C3B
• Synonyms: NT5C3B; NT5C3L; 5"-nucleotidase, cytosolic IIIB; 7-methylguanosine phosphate-specific 5"-nucleotidase; cN-III-like protein; cytosolic 5"-nucleotidase 3B; 7-methylguanosine nucleotidase; 5"-nucleotidase, cytosolic III-like; N(7)-methylguanylate 5"-phosphatase; cytosolic 5"-nucleotidase III-like protein; NP_443167.4; EC 3.1.3.5; EC 3.1.3.91
• Gene ID: 115024
• mRNA Refseq: NM_052935
• Protein Refseq: NP_443167
• UniProt ID: Q969T7
• Chromosome Location: 17q21.2
• Pathway: Nicotinate and nicotinamide metabolism; Purine metabolism; Pyrimidine metabolism
• Function: 5"-nucleotidase activity; magnesium ion binding; nucleotide binding