| Source : |
E. coli |
| Species : |
Rat |
| Tag : |
N/A |
| Form : |
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts.The ED50 for this effect is typically 0.05-0.3ng/mL. |
| Molecular Mass : |
The recombinant rat EGF consists of 54 amino acids and has a predicted molecular mass of 6.3 kDa. The apparent molecular mass of the recombinant protein is approximately 6-9 kDa in SDS-PAGE under reducing conditions. |
| Protein Length : |
Asn974-Arg1026 |
| Purity : |
> 95 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
