"RHOQ" Related Products

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Recombinant Human RHOQ 293 Cell Lysate

Cat.No.: RHOQ-2347HCL
Description: Antigen standard for ras homolog gene family, member Q (RHOQ) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RHOQ ras homolog family member Q [ Homo sapiens ]
Official Symbol: RHOQ
Synonyms: RHOQ; ras homolog family member Q; ARHQ, ras homolog gene family, member Q , RAS like, family 7, member A , RASL7A; rho-related GTP-binding protein RhoQ; TC10; ras-like protein TC10; RAS-like, family 7, member A; ras homolog gene family, member Q; ras-like protein family member 7A; ARHQ; TC10A; RASL7A;
Gene ID: 23433
mRNA Refseq: NM_012249
Protein Refseq: NP_036381
MIM: 605857
UniProt ID: P17081
Chromosome Location: 2p21
Pathway: Insulin Pathway, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem; Insulin-mediated glucose transport, organism-specific biosystem; Rho GTPase cycle, organism-specific biosystem; Signal Transduction, organism-specific biosystem;
Function: GBD domain binding; GTP binding; GTPase activity; nucleotide binding; profilin binding;

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