"PPP1R3C" Related Products

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Recombinant Human PPP1R3C 293 Cell Lysate

Cat.No.: PPP1R3C-2934HCL
Description: Antigen standard for protein phosphatase 1, regulatory (inhibitor) subunit 3C (PPP1R3C) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PPP1R3C protein phosphatase 1, regulatory subunit 3C [ Homo sapiens ]
Official Symbol: PPP1R3C
Synonyms: PPP1R3C; protein phosphatase 1, regulatory subunit 3C; PPP1R5, protein phosphatase 1, regulatory (inhibitor) subunit 3C; protein phosphatase 1 regulatory subunit 3C; Phosphatase 1; regulatory inhibitor subunit 5; protein targeting to glycogen; PTG; PP1 subunit R5; protein phosphatase 1 regulatory subunit 5; Phosphatase 1, regulatory inhibitor subunit 5; protein phosphatase 1, regulatory (inhibitor) subunit 3C; PPP1R5;
Gene ID: 5507
mRNA Refseq: NM_005398
Protein Refseq: NP_005389
MIM: 602999
UniProt ID: Q9UQK1
Chromosome Location: 10q23-q24
Pathway: Diurnally regulated genes with circadian orthologs, organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem;
Function: enzyme binding; protein binding; protein serine/threonine phosphatase activity;

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