"NSFL1C" Related Products

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Recombinant Human NSFL1C 293 Cell Lysate

Cat.No.: NSFL1C-3687HCL
Description: Antigen standard for NSFL1 (p97) cofactor (p47) (NSFL1C), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NSFL1C NSFL1 (p97) cofactor (p47) [ Homo sapiens ]
Official Symbol: NSFL1C
Synonyms: NSFL1C; NSFL1 (p97) cofactor (p47); NSFL1 cofactor p47; dJ776F14.1; p47; SHP1 homolog (S. cerevisiae); UBX domain protein 2C; UBX1; UBXD10; UBXN2C; SHP1 homolog; p97 cofactor p47; UBX domain-containing protein 2C; P47; MGC3347; FLJ46889;
Gene ID: 55968
mRNA Refseq: NM_001206736
Protein Refseq: NP_001193665
MIM: 606610
UniProt ID: Q9UNZ2
Chromosome Location: 20p13
Pathway: Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem;
Function: lipid binding;

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