Active Recombinant Human SPARCL1, His-tagged

• Cat.No.: SPARCL1-89H
• Product Overview: Recombinant human SPARCL1, Ile17-Phe664, fused with a C-terminal 10-His tag was expressed in Mouse myeloma cell line, NS0-derived

Specification

• Description: SPARCL1 (Secreted Protein, Acidic and Rich in Cysteines­like 1), also known as hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins. SPARCL1 is an anti­adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver. Human SPARCL1 contains a 16 amino acid (aa) signal sequence and a 648 aa mature region with four domains: a 416 aa N­terminal acidic region, a 23 aa follistatin­like domain, a 55 aa kazal­like segment and a 48 aa EF­hand/calcium­binding domain. SPARCL1 is predicted at 75 kDa, but migrates at ~130 kDa, which has been explained either by disulfide­linked homodimerization or by glycosylation and high acidity. Some truncated forms have been reported. In mouse, a 55 kDa C­terminal fragment is the only form in kidney and represents a portion of SPARCL1 in other tissues. In humans, a 25 kDa form is increased in liver tumors that are encapsulated, while the full­length form is downregulated in many epithelial cell­derived tumors. SPARCL1 inhibits adhesion and spreading on a variety of substrate. It is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma. In tonsillar high endothelial venules (HEV), SPARCL1 may induce endothelial cell dissociation, promoting extravasation. SPARCL1 binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin. Human mature SPARCL1 shares 67%, 69%, 78%, 76%, 72% and 72% aa identity with mouse, rat, equine, canine, porcine and bovine SPARCL1, respectively. The follistatin­like, kazal­like and calcium­binding domains of SPARCL1 show 61% aa identity with corresponding regions of SPARC.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ile17
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. Schiemann, B.J. et al. (2003) Mol. Biol. Cell. 14:3977.The ED50 for this effect is typically 0.5 - 2.0 µg/mL.Measured by its ability to bind Collagen I with an apparent KD < 5 nM.
• Molecular Mass: Predicted Molecular Mass: 74.9 kDaSDS-PAGE: 135-150 kDa, reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Avoid repeated freeze­thaw cycles.12 months from date of receipt, ­20 to ­ 70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after recons titution.3 months, ­20 to ­70 °C under sterile conditions after reconstitu tion.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: SPARCL1 SPARC-like 1 (hevin) [ Homo sapiens ]
• Official Symbol: SPARCL1
• Synonyms: SC1; MAST9; PIG33; MAST 9; SPARC-like protein 1; high endothelial venule protein; proliferation-inducing protein 33
• Gene ID: 8404
• MIM: 606041
• Chromosome Location: 4q22.1
• Function: calcium ion binding