| Cat.No. : |
CPE-210H |
| Product Overview : |
Recombinant Human CPE MS Standard Protein, C13 and N15-labeled (CPE, Heavy Labeled) Glu 26 - Ser 453 (Accession # NP_001864.1) was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain >99% incorporation efficien |
| Description : |
Carboxypeptidase E (CPE) is also known as Carboxypeptidase H or enkephalin convertase. Carboxypeptidase E cleaves C-terminal amino acid residues and is involved in neuropeptide processing. It is a peripheral membrane protein. CPE specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Carboxypeptidase E appears to have several functions. The active form of carboxypeptidase E was shown to be in secretory vesicles, where it acts as an exopeptidase to activate neuropeptides, It does that by cleaving off basic C-terminal amino acids, producing the active form of the peptide. Products of carboxypeptidase E include insulin, enkephalin, and most other neuroendocrine peptides.It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting signal for regulated secretory proteins in the trans-Golgi network of the pituitary and in secretory granules; regulated secretory proteins are mostly hormones and neuropeptides. Mice with mutant carboxypeptidase E Cpe, display endocrine disorders like obesity and infertility. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
| Molecular Mass : |
CPE, Heavy Labeled is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 49 kDa. The predicted N-terminus is Glu 26. DTT-reduced Protein migrates as 53 kDa in SDS-PAGE .CPE, Heavy Labeled is labeled with [U- 13C6, 15N4]-L-Arginine |
| Endotoxin : |
Less than 1.0 EU per μg of the CPE, Heavy Labeled by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC). |
| Reconstitution : |
See Certificate of Analysis for reconstitution instructions and specific concentrations. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.