Active Native Arachis hypogaea (peanut) Phospholipase D, Type II

• Cat.No.: PLD-18A
• Product Overview: Native Phospholipase D, Type II, was purified from Arachis hypogaea (peanut). CAS#9001-87-0

Specification

• Species: Arachis hypogaea
• Source: Arachis hypogaea
• Tag: Non
• Description: Phospholipase D is a phospholipid hydrolyzing enzyme and an important component of receptor-mediated signal transduction responses and regulated secretion. Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. Phospholipase D is involved in conferring drought susceptibility in peanuts, which increases the risk of aflatoxin contamination.
• Form: lyophilized powder. Partially purified, lyophilized powder containing buffer salts.
• Bio-activity: ≥60 units/mg protein
• Purity: Protein, ~30%
• Unit Definition: One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.
• Applications: Research has shown ADP-ribosylation factor regulation of phospholipase D is important in the release of nascent secretory vesicles from the trans-Golgi network. It has also been used in a study to investigate stimulation of Na+-Ca2+ exchange activity in canine cardiac sarcolemmal vesicals.
• Notes: Protein determined using biuret, unless otherwise indicated.
• Storage: Store at -20 °C