| Cat. No.: | Ubc9-14H |
| Product Overview: | Recombinant Human Ubiquitin Conjugating Enzyme 9 produced in E.coli is a 19.5 kDa protein containing 171 amino acids. The Ubc9 protein contains 6xHis tag and is purified by proprietary chromatographic techniques. |
| Description: | Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IκBα, and PML without the requirement of an E3 ligase. |
| Synonyms: | SUMO-conjugating enzyme UBC9; EC 6.3.2.-; SUMO-protein ligase; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; Ubiquitin carrier protein I; Ubiquitin carrier protein 9; p18; UBC9; C358B7.1; P18; UBCE9; SUMO-1-protein ligase; SUMO-protein ligase; ubiquitin carrier protein; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme E2I; ubiquitin-conjugating enzyme E2I (UBC9 homolog; yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I. |
| Source: | Escherichia Coli. |
| Amino acid sequence: | MHHHHHHAMGTLNMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIP GKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRP AITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS. |
| Physical Appearance: | Sterile Filtered white lyophilized powder. |
| Purity: | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.2 μm filtered concentrated (1 mg/ml) solution in 1X PBS and 1 mM DTT, pH 7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized Ubc9 in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Ubc9 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Ubc9 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Download Datasheet
See All Ubc9 Products
Download Datasheet