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Recombinant Mouse Grem1 Protein, His-tagged

Cat.No.: Grem1-1011M
Product Overview: Recombinant Mouse Grem1 (Accession # O70326) Lys25-Asp184, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Mouse
Tag: His
Predicted N Terminal: Lys25
Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Bio-activity: Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 0.025-0.15 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4.
Molecular Mass: Recombinant Mouse Grem1, His-tagged has a calculated MW of 19.6 kDa. In SDS-PAGE migrates as 23-31 kDa, reducing conditions.
Protein length: Lys25-Asp184
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: Grem1 gremlin 1 [ Mus musculus ]
Official Symbol: Grem1
Synonyms: GREM1; gremlin 1; gremlin-1; down-regulated in v-mos-transformed cells; cysteine knot superfamily 1, BMP antagonist 1; down-regulated in Mos-transformed cells protein; ld; Drm; Grem; Cktsf1b1;
Gene ID: 23892
mRNA Refseq: NM_011824
Protein Refseq: NP_035954
MIM:

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