"MMP14" Related Products


Recombinant Human MMP14 protein

Cat.No.: MMP14-1837H
Product Overview: Recombinant Human MMP14 (aa 89-265) was expressed in E. coli.
Description: MMP-14 is expressed in adult lung, placenta, kidney, ovaries, intestine, prostate and spleen. Increased amounts of the enzyme are found in tumor tissues as lung carcinoma, gastric caqrcinoma, colon, breast, head and neck carcinoma.MMP-14 is activated by removal of its prodomain. The reaction is catalyzed by furin, a subtilisin-type serine protease, which recognizes a motif of four basic amino acid residues located between prodomain and catalytic domain.MMP-14 activates pro-MMP-2 and pro-MMP-13 by proteolytic cleavage of their prodomains. The ability of MMP-14 to activate other MMPs provides potential for enhanced pericellular proteolysis in physiological and pathological processes. In particular, activation of pro-MMP-2 by MMP-14 is considered to contribute to local degradation of extracellular matrix during cell migration and proliferation. MMP-14 hydrolyzes also fibrillar collagens I, II and III into characteristic 3/4 and 1/4 fragments and it cleaves a number of other proteins of the extracellular matrix, among them fibronectin, vitronectin, laminin-1 and dermatan sulfate proteoglycan. The activity of MMP-14 is poorly inhibited by tissue inhibitor of matrix metalloproteinases-1 (TIMP-1), but efficiently inhibited by TIMP-2 and TIMP-3.
Source: E. coli
Species: Human
Form: Liquid. In 50mM TRIS-HCl, pH 7.5, 150mM NaCl, 5mM CaCl2.
Bio-activity: ≥140mU/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1µmol Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 per min. at 37 centigrade, pH 7.5.
Molecular Mass: ~20kDa (SDS-PAGE).
AA Sequence: Y89 A I Q G L K W Q H N E I T F C I Q N Y T P K V G E Y A T Y E A I R K A F R V W E S A T P L R F R E V P Y A Y I R E G H E K Q A D I M I F F A E G F H G D S T P F D G E G G F L A H A Y F P G P N I G G D T H F D S A E P W T V R N E D L N G N D I F L V A V H E L G H A L G L E H S S D P S A I M A P F Y Q W M D T E N F V L P D D D R R G I Q Q L Y G G E S G265
Purity: ≥95% (SDS-PAGE)
Applications: Used to study the activation of progelatinase A (matrix metalloproteinase 2) and the degradation of proteins of the extracellular matrix. The enzyme allows screening of matrix metalloproteinase inhibitors and characterization of inhibitor action.
Storage: Long Term Storage: -80 centigrade. Avoid freeze/thaw cycles.
Concentration: 0.2mg/ml
Gene Name: MMP14 matrix metallopeptidase 14 (membrane-inserted) [ Homo sapiens ]
Official Symbol: MMP14
Synonyms: MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase
Gene ID: 4323
mRNA Refseq: NM_004995
Protein Refseq: NP_004986
MIM: 600754
UniProt ID: P50281
Chromosome Location: 14q11.2
Pathway: AGE/RAGE pathway, organism-specific biosystem; Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem
Function: calcium ion binding; metalloendopeptidase activity; protein binding

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